Please use this identifier to cite or link to this item: https://apo.ansto.gov.au/dspace/handle/10238/6359
Title: Investigation of elemental changes in brain tissues following excitotoxic injury
Authors: Siegele, R
Howell, NR
Callaghan, PD
Pastuovic, Z
Keywords: PIXE analysis
Brain
Pathology
Animal tissues
Neurology
Ion microprobe analysis
Issue Date: 1-Jul-2013
Publisher: Elsevier
Citation: Siegele, R., Howell, N. R., Callaghan, P. D., & Pastuovic, Z. (2013). Investigation of elemental changes in brain tissues following excitotoxic injury. Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, 306, 125-128. doi:10.1016/j.nimb.2012.12.050
Abstract: Recently the ANSTO heavy ion microprobe has been used for elemental mapping of thin brain tissue sections. The fact that a very small portion of the proton energy is used for X-ray excitation combined with small variations of the major element concentrations makes μ-PIXE imaging and GeoPIXE analysis a challenging task. Excitotoxic brain injury underlies the pathology of stroke and various neurodegenerative disorders. Large fluxes in Ca+2 cytosolic concentrations are a key feature of the initiation of this pathophysiological process. In order to understand if these modifications are associated with changes in the elemental composition, several brain sections have been mapped with μ-PIXE. Increases in Ca+2 cytosolic concentrations were indicative of the pathophysiological process continuing 1 week after an initiating neural insult. We were able to measure significant variations in K and Ca concentration distribution across investigated brain tissue. These variations correlate very well with physiological changes visible in the brain tissue. Moreover, the obtained μ-PIXE results clearly demonstrate that the elemental composition changes significantly correlate with brain drauma. © 2013, Elsevier B.V.
Gov't Doc #: 6202
URI: http://dx.doi.org/10.1016/j.nimb.2012.12.050
http://apo.ansto.gov.au/dspace/handle/10238/6359
ISSN: 0168-583X
Appears in Collections:Journal Articles

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