Production and use of deuterated polyhydroxyoctanoate in structural studies of PHO inclusions
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Date
2007-11-01
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
This work reports on the biosynthesis of polyhydroxyalkanoates with medium chain length alkyl substituents in the side chain by Pseudomonas oleovorans using hydrogenated and deuterated substrates. These investigations aimed to obtain polyhydroxyalkanoates with varying degrees of deuterium substitution, and establish whether they are suitable analogues for structural investigation. In order to understand the formation and structure of inclusions in their native state, whole inclusions were isolated from microbial cells and were analysed using Small Angle Neutron Scattering. A contrast variation study was conducted on hydrogenated and deuterated inclusions of polyhydroxyoctanoate, as well as inclusions resulting from co-feeding or sequentially feeding different precursors. The data indicated a core/shell structure resulting from feeding hydrogenated followed by perdeuterated PHO precursor, and demonstrated the utility of this analysis for characterising chemically similar systems. © 2007, Elsevier Ltd.
Description
Keywords
Biosynthesis, Deuteration, Deuterium, Small angle scattering, Hydrogenation, Feeding
Citation
Russell, R. A., Holden, P. J., Wilde, K. L., Harnmerton, K. M., & Foster, L. J. R. (2007). Production and use of deuterated polyhydroxyoctanoate in structural studies of PHO inclusions. Journal of Biotechnology, 132(3), 303-305. doi:10.1016/j.jbiotec.2007.03.018