ANSTO Publications Online >
Journal Publications >
Journal Articles >

Please use this identifier to cite or link to this item: http://apo.ansto.gov.au/dspace/handle/10238/1066

Title: Production and use of deuterated polyhydroxyoctanoate in structural studies of PHO inclusions.
Authors: Russell, RA
Holden, PJ
Wilde, KL
Hammerton, KM
Foster, LJR
Keywords: Biosynthesis
Deuteration
Deuterium
Small Angle Scattering
Hydrogenation
Feeding
Issue Date: 1-Nov-2007
Publisher: Elsevier
Citation: Russell, R. A., Holden, P. J., Wilde, K. L., Harnmerton, K. M., & Foster, L. J. R. (2007). Production and use of deuterated polyhydroxyoctanoate in structural studies of PHO inclusions. Journal of Biotechnology, 132(3), 303-305.
Abstract: This work reports on the biosynthesis of polyhydroxyalkanoates with medium chain length alkyl substituents in the side chain by Pseudomonas oleovorans using hydrogenated and deuterated substrates. These investigations aimed to obtain polyhydroxyalkanoates with varying degrees of deuterium substitution, and establish whether they are suitable analogues for structural investigation. In order to understand the formation and structure of inclusions in their native state, whole inclusions were isolated from microbial cells and were analysed using Small Angle Neutron Scattering. A contrast variation study was conducted on hydrogenated and deuterated inclusions of polyhydroxyoctanoate, as well as inclusions resulting from co-feeding or sequentially feeding different precursors. The data indicated a core/shell structure resulting from feeding hydrogenated followed by perdeuterated PHO precursor, and demonstrated the utility of this analysis for characterising chemically similar systems. © 2007, Elsevier Ltd.
URI: http://dx.doi.org/10.1016/j.jbiotec.2007.03.018
http://apo.ansto.gov.au/dspace/handle/10238/1066
ISSN: 0168-1656
Appears in Collections:Journal Articles

Files in This Item:

There are no files associated with this item.

Items in APO are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback