Production and use of deuterated polyhydroxyoctanoate in structural studies of PHO inclusions

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dc.contributor.authorRussell, RAen_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorWilde, KLen_AU
dc.contributor.authorHammerton, KMen_AU
dc.contributor.authorFoster, LJRen_AU
dc.date.accessioned2008-04-14T03:58:04Zen_AU
dc.date.accessioned2010-04-30T05:07:24Zen_AU
dc.date.available2008-04-14T03:58:04Zen_AU
dc.date.available2010-04-30T05:07:24Zen_AU
dc.date.issued2007-11-01en_AU
dc.date.statistics2007-11en_AU
dc.description.abstractThis work reports on the biosynthesis of polyhydroxyalkanoates with medium chain length alkyl substituents in the side chain by Pseudomonas oleovorans using hydrogenated and deuterated substrates. These investigations aimed to obtain polyhydroxyalkanoates with varying degrees of deuterium substitution, and establish whether they are suitable analogues for structural investigation. In order to understand the formation and structure of inclusions in their native state, whole inclusions were isolated from microbial cells and were analysed using Small Angle Neutron Scattering. A contrast variation study was conducted on hydrogenated and deuterated inclusions of polyhydroxyoctanoate, as well as inclusions resulting from co-feeding or sequentially feeding different precursors. The data indicated a core/shell structure resulting from feeding hydrogenated followed by perdeuterated PHO precursor, and demonstrated the utility of this analysis for characterising chemically similar systems. © 2007, Elsevier Ltd.en_AU
dc.identifier.citationRussell, R. A., Holden, P. J., Wilde, K. L., Harnmerton, K. M., & Foster, L. J. R. (2007). Production and use of deuterated polyhydroxyoctanoate in structural studies of PHO inclusions. Journal of Biotechnology, 132(3), 303-305. doi:10.1016/j.jbiotec.2007.03.018en_AU
dc.identifier.govdoc1474en_AU
dc.identifier.issn0168-1656en_AU
dc.identifier.issue3en_AU
dc.identifier.journaltitleJournal of Biotechnologyen_AU
dc.identifier.pagination303-305en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.jbiotec.2007.03.018en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/1066en_AU
dc.identifier.volume132en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectBiosynthesisen_AU
dc.subjectDeuterationen_AU
dc.subjectDeuteriumen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectHydrogenationen_AU
dc.subjectFeedingen_AU
dc.titleProduction and use of deuterated polyhydroxyoctanoate in structural studies of PHO inclusionsen_AU
dc.typeJournal Articleen_AU
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