Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials.
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Date
2010-06-01
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Abstract
Mutually complementary, self-repulsive oligopeptide pairs were designed to coassemble into viscoelastic hydrogels. Peptide engineering was combined with biophysical techniques to investigate the effects of temperature on the structural and mechanical properties of the resulting hydrogels. Biophysical characterizations, including dynamic rheometry, small-angle X-ray scattering (SAXS), and fluorescence spectroscopy, were used to investigate hydrogelation at the bulk, fiber, and molecular levels, respectively. It has been found that temperature has a significant effect on the structure and mechanical properties of peptide-based biomaterials. Oligopeptide fibers assembled at 25°C are formed faster and are two times thicker, and the resulting material is mechanically seven times stronger than that assembled at 5°C. © 2010, American Chemical Society
Description
Keywords
Hydrogels, Peptides, Temperature range, Mechanical properties, Small angle scattering, Fluorescence spectroscopy
Citation
Ramachandran, S., Taraban, M. B., Trewhella, J., Gryczynski, I., Gryczynski, Z., & Yu, Y. B. (2010). Effect of temperature during assembly on the structure and mechanical properties of peptide-based materials. Biomacromolecules, 11(6), 1502-1506. doi:10.1021/bm100138m