Phytantriol-based cubosome formulation as an antimicrobial against Lipopolysaccharide-deficient gram-gegative bacteria
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Date
2020-09-17
Journal Title
Journal ISSN
Volume Title
Publisher
American Chemical Society
Abstract
Treatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials. Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade. In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains. Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes. Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support. These results reveal that cubosomes might be a new strategy for combating LPS-deficient Gram-negative pathogens. © 2020 American Chemical Society.
Description
Keywords
Antimicrobial agents, Lipopolysaccharides, Bacteria, Lipids, Membranes, Neutron diffraction, Nanotechnology
Citation
Lai, X., Ding, Y., Wu, C.-M., Chen, X., Jiang, J.-h., Hsu, H.-Y., Wang, Y., Le Brun, A. P., Song, J., Han, M.-L., Li, J., & Shen, H.-H. (2020). Phytantriol-based cubosome formulation as an antimicrobial against Lipopolysaccharide-deficient gram-gegative bacteria. ACS Applied Materials & Interfaces, 12(40), 44485-44498. doi:10.1021/acsami.0c13309