Phytantriol-based cubosome formulation as an antimicrobial against Lipopolysaccharide-deficient gram-gegative bacteria

Simple item page
dc.contributor.authorLai, XFen_AU
dc.contributor.authorDing, Yen_AU
dc.contributor.authorWu, CMen_AU
dc.contributor.authorChen, Xen_AU
dc.contributor.authorJiang, JHen_AU
dc.contributor.authorHsu, HYen_AU
dc.contributor.authorWang, Yen_AU
dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorSong, JNen_AU
dc.contributor.authorHan, MLen_AU
dc.contributor.authorLi, Jen_AU
dc.contributor.authorShen, HHen_AU
dc.date.accessioned2024-12-12T22:00:50Zen_AU
dc.date.available2024-12-12T22:00:50Zen_AU
dc.date.issued2020-09-17en_AU
dc.date.statistics2024-12-04en_AU
dc.description.abstractTreatment of multidrug-resistant (MDR) bacterial infections increasingly relies on last-line antibiotics, such as polymyxins, with the urgent need for discovery of new antimicrobials. Nanotechnology-based antimicrobials have gained significant importance to prevent the catastrophic emergence of MDR over the past decade. In this study, phytantriol-based nanoparticles, named cubosomes, were prepared and examined in vitro by minimum inhibitory concentration (MIC) and time-kill assays against Gram-negative bacteria: Acinetobacter baumannii, Klebsiella pneumoniae, and Pseudomonas aeruginosa. Phytantriol-based cubosomes were highly bactericidal against polymyxin-resistant, lipopolysaccharide (LPS)-deficient A. baumannii strains. Small-angle neutron scattering (SANS) was employed to understand the structural changes in biomimetic membranes that replicate the composition of these LPS-deficient strains upon treatment with cubosomes. Additionally, to further understand the membrane-cubosome interface, neutron reflectivity (NR) was used to investigate the interaction of cubosomes with model bacterial membranes on a solid support. These results reveal that cubosomes might be a new strategy for combating LPS-deficient Gram-negative pathogens. © 2020 American Chemical Society.en_AU
dc.description.sponsorshipThe authors gratefully acknowledge the use of the facility and the assistance of Jisheng Ma at the Monash X-ray Platform (Monash University, Australia). They thank Heidi Yu and Hasini Wickremasinghe for their help in in vitro experiment, and Eric Hanssen from the Bio21 Advanced Microscopy Facility (The University of Melbourne, Australia) for his help in Cryo-TEM. The Australian Nuclear Science and Technology Organization (ANSTO) is gratefully acknowledged for providing the neutron research facilities (proposals P6945 and P8274). H.-H.S. is an Australian NHMRC Career Development Research Fellow (GNT1106798). This work was supported by the NHMRC project grant (APP1144652).en_AU
dc.format.mediumPrint-Electronicen_AU
dc.identifier.citationLai, X., Ding, Y., Wu, C.-M., Chen, X., Jiang, J.-h., Hsu, H.-Y., Wang, Y., Le Brun, A. P., Song, J., Han, M.-L., Li, J., & Shen, H.-H. (2020). Phytantriol-based cubosome formulation as an antimicrobial against Lipopolysaccharide-deficient gram-gegative bacteria. ACS Applied Materials & Interfaces, 12(40), 44485-44498. doi:10.1021/acsami.0c13309en_AU
dc.identifier.issn1944-8244en_AU
dc.identifier.issn1944-8252en_AU
dc.identifier.issue40en_AU
dc.identifier.journaltitleACS Applied Materials & Interfacesen_AU
dc.identifier.pagination44485-44498en_AU
dc.identifier.urihttps://doi.org/10.1021/acsami.0c13309en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15815en_AU
dc.identifier.volume12en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.subjectAntimicrobial agentsen_AU
dc.subjectLipopolysaccharidesen_AU
dc.subjectBacteriaen_AU
dc.subjectLipidsen_AU
dc.subjectMembranesen_AU
dc.subjectNeutron diffractionen_AU
dc.subjectNanotechnologyen_AU
dc.titlePhytantriol-based cubosome formulation as an antimicrobial against Lipopolysaccharide-deficient gram-gegative bacteriaen_AU
dc.typeJournal Articleen_AU
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
am0c13309_si_001.pdf
Size:
998.9 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.66 KB
Format:
Plain Text
Description:
Download
Collections
Journal Articles