Uptake of boronated monoclonal antibodies by melanoma cells visualised by track etch autoradiography and electron energy loss spectroscopy.

Moore, DE; Stretch, JR; Dawes, AL; Cockayne, DJH; Allen, BJ; Constantine, G

Uptake of boronated monoclonal antibodies by melanoma cells visualised by track etch autoradiography and electron energy loss spectroscopy.

Date

1992

Authors

Publisher

Plenum Press

Abstract

The monoclonal antibody (MAb) NKI-C3 (Netherlands Cancer Institute) is a murine IgGl known to react with a formalin resistant antigen expressed by more than 95% of human melanomas. In principle, it can be used in the diagnosis of tissue sections for the presence of melanocytes, and with the appropriate level of boron labelling, this antibody could also be used for NCT of melanoma, provided its specificity is not impaired by the labelling process. The present study involves the use of two imaging techniques: first, α-track etch autoradiography to determine the specifiCity of the MAb after boronation, and second, electron energy loss spectroscopy to obtain information at the subcellular level concerning the localisation of the boron label. © Plenum Press

Description

Physical copy held by ANSTO Library at DDC: 616.994/17

Keywords

Boron, Antibodies, Melanomas, Autoradiography, Energy-loss spectroscopy, Netherlands, Neoplasms, Achromatic Lesions, Cold neutrons, Scattering

Citation

Moore, D. E., Stretch, J. R., Dawes, A. L., Cockayne, D. J. H., Allen, B. J., & Constantine, G. (1992). Uptake of boronated monoclonal antibodies by melanoma cells visualised by track etch autoradiography and electron energy loss spectroscopy. Paper presented to the Fourth International Symposium on Neutron Capture Therapy for Cancer, December 4-7, 1990, Sydney, Australia. In B. J. Allen, D. E. Moore, & B. V. Harrington (Eds.), Progress in Neutron Capture Therapy for Cancer (pp. 335-338). New York, USA: Plenum Press.