Histological examination of melanoma xenografts in the nude mouse model: pre and post neutron capture therapy

Crotty, K; Mallesch, J; Moore, DE; Allen, BJ

Histological examination of melanoma xenografts in the nude mouse model: pre and post neutron capture therapy

Date

1991-10-02

Authors

Publisher

AINSE

Abstract

Following the inoculation of 1.5 million Harding Passey melanoma cells in the thigh, subcutaneous (sc) murine melanoma xenografts were grown in the nude mouse model. Mice received 12 mg of enriched boron-10 boronophenylalanine (10BPA ; NCT mice) or no BPA (neutrons only, mice) and the tumours exposed to a neutron fluence of 1013 n cm-2 four hours post ip injection (1). Tumours in the neutrons only mice regressed but all recurred, showing a growth delay of about 3 weeks. However, only 1 of 8 NCT mice showed short term recurrence and 1 of 7 long term recurrence. Three of the NCT mice lived in excess of 300 days before being sacrificed. The irradiated tumour beds showed a dense black mass still visible under the skin but no tumour growth was observed. Sections of the residual NCT tumours, of tumours treated with neutrons only and of untreated tumours were made and stained with haematoxylin and eosin (H&E). No mitoses were observed in the NCT sections, whereas these are evident in all untreated tumours. However, pleomorphic cells were observed in close proximity to capillaries and surrounded by cells with the appearance of histiocytes containing brown pigment. These cells are being examined by other techniques such as electron microscopy to determine whether or not the^ are melanoma cells.

Description

Physical copy held by ANSTO Library at DDC: 571.45/15

Keywords

Biological recovery, Boron 10, Experimental neoplasms, Histology, Melanomas, Mice, Neutron capture therapy, Post-irradiation examination, Radiotherapy, Therapy

Citation

Crotty, K., Mallesch, J., Moore, D. E., & Allen, B. J., (1991). Histological examination of melanoma xenografts in the nude mouse model: pre and post neutron capture therapy. Presentation to the 13th AINSE Radiation Biology Conference, 2-4 October 1991, Lucas Heights - AINSE Theatre : conference handbook (programme, abstracts and general information), (pp. 53). Lucas Heights, New South Wales : AINSE.