Deuterated phospholipids to study the structure, function and dynamics of membrane proteins using neutron scattering
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Date
2021-11-26
Authors
Journal Title
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Publisher
Australian Nuclear Science and Technology Organisation
Abstract
Contrast matching and contrast variation in neutron scattering provide unparalleled power for understanding the structure, function, and dynamics of a selected component in a multicomponent system. A sophisticated contrast study often requires the availability of deuterated molecules in which deuterium atoms are introduced in a predictable and controlled fashion to replace protons. This can be achieved by direct deuteration of precursors followed by custom chemical synthesis, for which expertise and capabilities have been developed at facility (NDF), ANSTO. It this paper we will discuss recent high impact research output using deuterated phospholipids produced by NDF/ANSTO. We will describe the synthesis and applications of selectively or perdeuterated unsaturated phospholipids to contrast match out the whole lipid bilayer or nano disks within a multicomponent system. Further, we also describe their role in investigations related to membrane lipoproteins (ApoE) exchange in relation to lipid unsaturation,[1] effect of membrane composition,[2] and conformational analysis Mg+2 channel by neutron scattering techniques.[2, 3]
Description
Citation shows N. R Yepuri as R. Yepuri.
Keywords
Deuteration, Phospholipids, Membranes, Scattering, Molecules, Protons, Synthesis, Deuterium
Citation
Yepuri, R., Moir, M, Krause-Heuer, A., & Darwish, T. (2021). Deuterated phospholipids to study the structure, function and dynamics of membrane proteins using neutron scattering. Presentation to the ANSTO User Meeting, Online, 24-26 November 2021. Retrieved from: https://events01.synchrotron.org.au/event/146/contributions/4290/contribution.pdf