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Title: Localisation of trace metals in hyperaccumulating plants using μ-PIXE
Authors: Siegele, R
Kachenko, AG
Wang, YD
Ionescu, M
Bhatia, NP
Cohen, DD
Keywords: PIXE analysis
Plant tissues
Issue Date: Nov-2007
Citation: Siegele, R., Kachenko, A. G., Wang, Y. D., Ionescu, M., Bhatia, N. P., & Cohen, D. D. (2007). Localisation of trace metals in hyperaccumulating plants using μ-PIXE. 15th Australian Conference on Nuclear and Complementary Techniques of Analysis and 9th Vacuum Society of Australia Congress, 21st – 23rd November 2007. Melbourne, Australia: University of Melbourne.
Abstract: PIXE is a very sensitive technique that can fast and reliably measure a wide range of elements simultaneously with high sensitivity. Using a focused microbeam elemental distributions can be mapped with high spatial resolution. We demonstrate high resolution mapping of metals in plant leafs at 5 um resolution and it's application in detecting sites of metal accumulation in metal-accumulating plant tissues. The importance of biological sample preparation is discussed by direct comparison of freeze-substitution and freeze-drying routinely used in biological sciences. The advantages and limitations of quantitative elemental imaging using these techniques are also discussed.
Gov't Doc #: 1016
Appears in Collections:Conference Publications

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