Deuterium effects in human serum albumin with nanoparticle silica kinetics
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Date
2016-11-29
Authors
White, JW
Raynes, JK
Mata, JP
Gilbert, EP
Knott, RB
de Campo, L
Journal Title
Journal ISSN
Volume Title
Publisher
Australian Institute of Nuclear Science and Engineering
Abstract
Light scattering shows three stages of the interaction of 80Å radius silica nanoparticles with human serum albumin in buffered solutions. The structures formed in the fast stage, twenty minutes after mixing, have been identified in a “stopped flow” neutron small angle scattering experiment. Good scattering functions were obtained at two-minute time resolution for this phase of the interaction in D2O and H2O. pH dependent changes in structure are analysed using standard fitting programs with a minimum number of parameters.
This experiment was aimed to find the structural signature of nanoparticle-protein interaction, possibly the “protein corona” supposed to be formed as a means to promote entry of nanoparticles into cells. Here we use small “engineered” nanoparticles where the indications of toxicity are strong. We show with nanometric resolution that for our system, the association is largely a form of protein-induced aggregation distinct from the protein corona hypothesis. The corona might well be the mode of interaction for small proteins and
nanoparticles 10-100 times larger than we have studied, but measurements on widely used commercial products may be more relevant.
Description
Keywords
Nanoparticles, Light scattering, Albumins, Small angle scattering, Proteins, Toxicity
Citation
White, J., Raynes, J., Mata, J. P., Gilbert, E., Knott, R., & de Campo, L. (2016). Deuterium effects in human serum albumin with nanoparticle silica kinetics. Paper presented at 13th AINSE-ANBUG Neutron Scattering Symposium, Sydney, NSW, Australia, 29-30 November 2016.