Deuterium effects in human serum albumin with nanoparticle silica kinetics

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dc.contributor.authorWhite, JWen_AU
dc.contributor.authorRaynes, JKen_AU
dc.contributor.authorMata, JPen_AU
dc.contributor.authorGilbert, EPen_AU
dc.contributor.authorKnott, RBen_AU
dc.contributor.authorde Campo, Len_AU
dc.date.accessioned2021-10-26T21:51:39Zen_AU
dc.date.available2021-10-26T21:51:39Zen_AU
dc.date.issued2016-11-29en_AU
dc.date.statistics2021-10-11en_AU
dc.description.abstractLight scattering shows three stages of the interaction of 80Å radius silica nanoparticles with human serum albumin in buffered solutions. The structures formed in the fast stage, twenty minutes after mixing, have been identified in a “stopped flow” neutron small angle scattering experiment. Good scattering functions were obtained at two-minute time resolution for this phase of the interaction in D2O and H2O. pH dependent changes in structure are analysed using standard fitting programs with a minimum number of parameters. This experiment was aimed to find the structural signature of nanoparticle-protein interaction, possibly the “protein corona” supposed to be formed as a means to promote entry of nanoparticles into cells. Here we use small “engineered” nanoparticles where the indications of toxicity are strong. We show with nanometric resolution that for our system, the association is largely a form of protein-induced aggregation distinct from the protein corona hypothesis. The corona might well be the mode of interaction for small proteins and nanoparticles 10-100 times larger than we have studied, but measurements on widely used commercial products may be more relevant.en_AU
dc.identifier.citationWhite, J., Raynes, J., Mata, J. P., Gilbert, E., Knott, R., & de Campo, L. (2016). Deuterium effects in human serum albumin with nanoparticle silica kinetics. Paper presented at 13th AINSE-ANBUG Neutron Scattering Symposium, Sydney, NSW, Australia, 29-30 November 2016.en_AU
dc.identifier.conferenceenddate30 November 2016en_AU
dc.identifier.conferencename13th AINSE-ANBUG Neutron Scattering Symposiumen_AU
dc.identifier.conferenceplaceSydney, NSW, Australiaen_AU
dc.identifier.conferencestartdate29 November 2016en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/12101en_AU
dc.language.isoenen_AU
dc.publisherAustralian Institute of Nuclear Science and Engineeringen_AU
dc.subjectNanoparticlesen_AU
dc.subjectLight scatteringen_AU
dc.subjectAlbuminsen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectProteinsen_AU
dc.subjectToxicityen_AU
dc.titleDeuterium effects in human serum albumin with nanoparticle silica kineticsen_AU
dc.typeConference Abstracten_AU
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