Synchrotron-based imaging reveals the fate of selenium in striped marsh frog tadpoles
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Date
2021-08-12
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American Chemical Society
Abstract
Synchrotron-based X-ray fluorescence microscopy (XFM) coupled with X-ray absorption near-edge structure (XANES) imaging was used to study selenium (Se) biodistribution and speciation in Limnodynastes peronii tadpoles. Tadpoles were exposed to dissolved Se (30 μg/L) as selenite (SeIV) or selenate (SeVI) for 7 days followed by 3 days of depuration. High-resolution elemental maps revealed that Se partitioned primarily in the eyes (specifically the eye lens, iris, and retinal pigmented epithelium), digestive and excretory organs of SeIV-exposed tadpoles. Speciation analysis confirmed that the majority of accumulated Se was converted to organo-Se. Multielement analyses provided new information on Se colocalization and its impact on trace element homeostasis. New insights into the fate of Se on a whole organism scale contribute to our understanding of the mechanisms and risks associated with Se pollution. © 2021 American Chemical Society
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Keywords
Amphibians, Biological accumulation, Multi-element analysis, Spectroscopy, X-ray fluorescence analysis, Anatomy, Selenium, Selenium compounds
Citation
Lanctôt, C. M., Cresswell, T., Lombi, E., & Bennett, W. W. (2021). Synchrotron-based imaging reveals the fate of selenium in striped marsh frog tadpoles. Environmental Science & Technology, 55(17), 11848-11858. doi:10.1021/acs.est.1c02028