A study of the interaction between polyelectrolytes and biomolecules at the solid/solution interface with neutron specular reflectivity
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Date
2017-07-12
Journal Title
Journal ISSN
Volume Title
Publisher
International Conference on Neutron Scattering
Abstract
Cellulose fibres may be formed into a low cost substrate, paper, for the adsorption of biomolecules to form the basis of cheap robust diagnostic tests. Important issues for the development of this technology are the retention of the biomolecules and maintaining the activity of the absorbed biomolecule. Typically the large internal surface is rough and there are limited experimental techniques to directly examine the structure of the adsorbed layer. In this work we describe our progress in using neutron and x-ray reflectivity to study the interaction between the cellulose surface and various adsorbed biomolecules with the aim to produce a model system to provide fundamental understanding for the production of robust paper based sensors. We have produced flat cellulose films from biodeuterated cellulose of varying degrees of non-exchangeable deuteration using bacterial cellulose produced with deuterated carbon sources. The films are amorphous with the fully hydrated films exhibiting 100% exchangeability of hydroxyl cellulose groups. The enhanced contrast, with respect to protein in particular, will allow visualisation of this class of molecules on the surface. The films are suitable for the visualisation of the diffuseness of a layer of cationic polyelectrolyte adsorbed to a cellulose surface. Giving, in principle,control on the charge sign and density of the cellulose surface for adsorption of biomolecules.
Description
Keywords
Adsorption, Cellulose, X-ray diffraction, Neutron diffraction, Paper, Deuteration, Films, Amorphous state
Citation
Garvey, C., Raghuwanshi, V. S., Su, J., Raverty, W., Holt, S. A., Holden, P. J., & Garnier, G. (2017). A study of the interaction between polyelectrolytes and biomolecules at the solid/solution interface with neutron specular reflectivity. Paper presented at ICNS 2017 (International Conference on Neutron Scattering), Daejeon, South Korea, 9 to 13 July 2017. Retrieved from: http://www.icns2017.org/program.php