A study of the interaction between polyelectrolytes and biomolecules at the solid/solution interface with neutron specular reflectivity

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dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorRaghuwanshi, VSen_AU
dc.contributor.authorSu, Jen_AU
dc.contributor.authorRaverty, Wen_AU
dc.contributor.authorHolt, SAen_AU
dc.contributor.authorHolden, PJen_AU
dc.contributor.authorGarnier, Gen_AU
dc.date.accessioned2021-08-09T22:28:12Zen_AU
dc.date.available2021-08-09T22:28:12Zen_AU
dc.date.issued2017-07-12en_AU
dc.date.statistics2021-08-09en_AU
dc.description.abstractCellulose fibres may be formed into a low cost substrate, paper, for the adsorption of biomolecules to form the basis of cheap robust diagnostic tests. Important issues for the development of this technology are the retention of the biomolecules and maintaining the activity of the absorbed biomolecule. Typically the large internal surface is rough and there are limited experimental techniques to directly examine the structure of the adsorbed layer. In this work we describe our progress in using neutron and x-ray reflectivity to study the interaction between the cellulose surface and various adsorbed biomolecules with the aim to produce a model system to provide fundamental understanding for the production of robust paper based sensors. We have produced flat cellulose films from biodeuterated cellulose of varying degrees of non-exchangeable deuteration using bacterial cellulose produced with deuterated carbon sources. The films are amorphous with the fully hydrated films exhibiting 100% exchangeability of hydroxyl cellulose groups. The enhanced contrast, with respect to protein in particular, will allow visualisation of this class of molecules on the surface. The films are suitable for the visualisation of the diffuseness of a layer of cationic polyelectrolyte adsorbed to a cellulose surface. Giving, in principle,control on the charge sign and density of the cellulose surface for adsorption of biomolecules.en_AU
dc.identifier.citationGarvey, C., Raghuwanshi, V. S., Su, J., Raverty, W., Holt, S. A., Holden, P. J., & Garnier, G. (2017). A study of the interaction between polyelectrolytes and biomolecules at the solid/solution interface with neutron specular reflectivity. Paper presented at ICNS 2017 (International Conference on Neutron Scattering), Daejeon, South Korea, 9 to 13 July 2017. Retrieved from: http://www.icns2017.org/program.phpen_AU
dc.identifier.conferenceenddate13 July 2017en_AU
dc.identifier.conferencenameICNS 2017 (International Conference on Neutron Scattering)en_AU
dc.identifier.conferenceplaceDaejeon, South Koreaen_AU
dc.identifier.conferencestartdate9 July 2017en_AU
dc.identifier.urihttp://www.icns2017.org/program.phpen_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/11284en_AU
dc.language.isoenen_AU
dc.publisherInternational Conference on Neutron Scatteringen_AU
dc.subjectAdsorptionen_AU
dc.subjectCelluloseen_AU
dc.subjectX-ray diffractionen_AU
dc.subjectNeutron diffractionen_AU
dc.subjectPaperen_AU
dc.subjectDeuterationen_AU
dc.subjectFilmsen_AU
dc.subjectAmorphous stateen_AU
dc.titleA study of the interaction between polyelectrolytes and biomolecules at the solid/solution interface with neutron specular reflectivityen_AU
dc.typeConference Abstracten_AU
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