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Langmuir-Schaefer deposition to create an asymmetrical lipopolysaccharide sparsely tethered lipid bilayer
(Springer Nature, 2021-12) Cranfield, CG; Le Brun, AP; García, ÁL; Cornell, BA; Holt, SA
Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182–189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers. © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Development of a modular ceramic knee prosthesis
(Humana Press, 2000) Payten, WM; Ben-Nissan, B
Degenerative joint disease, recognized as an increasing problem for society, is a direct result of an aging population (1). When patients present with joint pain, their primary concern is the relief of pain and return to a mobile life style. This often requires replacement of skeletal parts, such as hips, knees, elbows, finger joints, shoulder, and teeth, or fusion of vertebrae, and repair or augmentation of the jaw and bones of the skull. The result is a current worldwide orthopedic market valued at over $5 billion; joint replacement represents 68% of this market. The demand for knee replacements is increasing at approx 17%/yr, with some 300,000 knee joints replaced each year in the United States alone (2). This increase results in part from increased confidence in using such prostheses. Unfortunately, results do not reinforce this confidence: Long-term clinical results are scattered (3), and, although the overall rate of failure is reasonably low, it remains unacceptable. A further complication arises because the increase in younger patients undergoing total knee arthroplasty (TKA) may well lead to a higher incidence of eventual failure. © Springer Science+Business Media New York 2000
Deuteration for biological SANS: case studies, success and challenges in chemistry and biology
(Elsevier, 2022-11) Duff, AP; Cagnes, MP; Darwish, TA; Krause-Heuer, AM; Moir, M; Recsei, C; Rekas, A; Russell, RA; Wilde, KL; Yepuri, NR
Small angle neutron scattering is a powerful complementary technique in structural biology. It generally requires, or benefits from, deuteration to achieve its unique potentials. Molecular deuteration has become a mature expertise, with deuteration facilities located worldwide to support access to the technique for a wide breadth of structural biology and life sciences. The sorts of problems well answered by small angle scattering and deuteration involve large (> 10 Å) scale flexible movements, and this approach is best used where high-resolution methods (crystallography, NMR, cryo-EM) leave questions unanswered. This chapter introduces deuteration, reviewing biological deuteration of proteins, lipids and sterols, and then steps through the ever-expanding range of deuterated molecules being produced by chemical synthesis and enabling sophisticated experiments using physiologically relevant lipids. Case studies of recent successful use of deuteration may provide illustrative examples for strategies for future experiments. We discuss issues of nomenclature for synthesised molecules of novel labeling and make recommendations for their naming. We reflect on our experiences, with cost associated with achieving an arbitrary deuteration level, and on the benefits of experimental co-design by user scientist, deuteration scientist, and neutron scattering scientist working together. Although methods for biological and chemical deuteration are published in the public domain, we recommend that the best method to deuterate is to engage with a deuteration facility. © 2022 Elsevier
Using polarized neutron reflectometry to resolve effects of light elements and ion exposure on magnetization
(Elsevier, 2020) Callori, SJ; Saerbeck, T; Cortie, DL; Lin, KW
This chapter introduces the polarized neutron reflectometry (PNR) technique with a focus on its unique applications to studying the effects of light elements and ion beams in magnetic thin films. The chapter is divided into six sections. Following a brief introduction in Section 1, Section 2 introduces the operational principles and advantages of PNR. Section 3 discusses recent experiments on magnetic hydrogen sensors using in-situ magnetic measurements made on a PNR beam line. Section 4 reviews recent progress using PNR to clarify how low-energy ion beams can modulate the magnetic properties by implantation, modifying oxygen stoichiometry, interface engineering with argon, and imprinting magnetic domains by driving phase transitions. Section 5 exemplifies how PNR can be used to study lateral magnetic domain structures patterned using helium ion beams. Section 6 presents conclusions and future perspectives in form of a brief roadmap highlighting some of the latest developments in PNR, and the new technical possibilities that are anticipated over the coming decade. © 2020 Elsevier Inc.
Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques
(Elsevier, 2015) Russell, RA; Garvey, CJ; Darwish, TA; Foster, LJR; Holden, PJ
The use of microbial biosynthesis to produced deuterated recombinant proteins is a well-established practice in investigations of the relationship between molecular structure and function using neutron scattering and nuclear magnetic resonance spectroscopy. However, there have been few reports of using microbial synthetic capacity to produce labeled native biopolymers. Here, we describe methods for the production of deuterated polyhydroxyalkanoate biopolyesters in bacteria, the polysaccharide chitosan in the yeast Pichia pastoris, and cellulose in the bacterium Gluconacetobacter xylinus. The resulting molecules offer not only multiple options in creating structural contrast in polymer blends and composites in structural studies but also insight into the biosynthetic pathways themselves. © 2015 Elsevier Inc.

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