Langmuir-Schaefer deposition to create an asymmetrical lipopolysaccharide sparsely tethered lipid bilayer
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Date
2021-12
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Nature
Abstract
Because they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182–189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers. © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Description
Keywords
Lipids, Lipopolysaccharides, Metals, Substrates, Bacteria, Deposition
Citation
Cranfield, C. G., Le Brun, A. P., Garcia, A., Cornell, B. A., & Holt, S. A. (2022). Langmuir-Schaefer deposition to create an asymmetrical lipopolysaccharide sparsely tethered lipid bilayer. In C. G. Cranfield (Ed.), Membrane Lipids: Methods and Protocols, (pp. 21-30). Humana, New York : Springer. doi:10.1007/978-1-0716-1843-1_3