Morphological changes in human melanoma cells following irradiation with thermal neutrons

Barkala, DK; Allen, BJ; Brown, JK; Mountford, M; Mishima, Y; Ichihashi, M

Morphological changes in human melanoma cells following irradiation with thermal neutrons

Date

1989

Authors

Publisher

Alan R. Liss, Inc

Abstract

Morphological changes in two human melanoma cell lines, MM96 and MM418, following irradiation with thermal neutrons, were studied using light and electron microscopy. The results show that the response of human malignant melanoma cells to neutron irradiation is both cell line dependent and dose dependent, and that in any given cell line, some cells are more resistant to irradiation than others, thus demonstrating heterogeneity in respect to radiosensitivity. Cells repopulating MM96 flasks after irradiation were morphologically similar to the cells of origin whereas in MM418 flasks cells differentiated into five morphologically distinct subgroups and showed increased melanization. The results also show that radiation causes distinctive morphological patterns of damage although ultrastructural changes unique to the high LET particles released from boron 10 neutron capture are yet to be identified. © Alan R. Liss Inc.

Description

Physical copy held by ANSTO Library at DDC: 616.99477/3 Also held as part of a journal Pigment Cell Research Vol. 2, No. 4 (1989) at the same DDC number.

Keywords

Amino acids, Animal cells, Animals, Aromatics, Boron compounds, Dose-response relationships, Electron microscopy, Melanomas, Neutron capture therapy, Neutrons, Phenylalanine, RBE, Tumor cells, Ultrastructural changes, Radiotherapy, Therapy

Citation

Barkla, D. H., Allen, B. J., Brown, J. K., Mountford, M., Mishima, Y., & Ichihashi, M. (1989). Morphological changes in human melanoma cells following irradiation with thermal neutrons. Paper prsented to the 2nd Japan-Australia International Workshop on Thermal Neutron Capture Therapy for Malignant Melanoma, Kobe, September 23-26, 1987. In Mishima, Y. (ed.). Melanoma neutron capture therapy : proceedings of 2nd Japan-Australia International Workshop on Thermal Neutron Capture Therapy for Malignant Melanoma, Kobe, September 23-26, 1987, (pp. 345-348). New York : Alan R. Liss.