An16-resilin: An advanced multi-stimuli-responsive resilin-mimetic protein polymer
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Date
2014-11-01
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
Engineered protein polymers that display responsiveness to multiple stimuli are emerging as a promising class of soft material with unprecedented functionality. The remarkable advancement in genetic engineering and biosynthesis has created the opportunity for precise control over the amino acid sequence, size, structure and resulting functions of such biomimetic proteins. Herein, we describe the multi-stimuli-responsive characteristics of a resilin-mimetic protein, An16-resilin (An16), derived from the consensus sequence of resilin gene in the mosquito Anopheles gambiae. We demonstrate that An16 is an intrinsically disordered protein that displays unusual dual-phase thermal transition behavior along with responsiveness to pH, ion, light and humidity. Identifying the molecular mechanisms that allow An16 to sense and switch in response to varying environments furthers the ability to design intelligent biomacromolecules. © 2014 Acta Materialia Inc.
Description
Keywords
Proteins, Polymers, Genetic engineering, Amino acids, Molecules
Citation
Balu, R., Dutta, N. K., Choudhury, N. R., Elvin, C. M., Lyons, R. E., Knott, R., & Hill, A. J. (2014). An16-resilin: An advanced multi-stimuli-responsive resilin-mimetic protein polymer. Acta Biomaterialia, 10(11), 4768-4777. doi:10.1016/j.actbio.2014.07.030