Studies on 99mtc-pertechnetate from the MEK solvent extraction generator
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Date
1984-12
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Publisher
Australian Atomic Energy Commission
Abstract
Analysis by gas chromatography-mass spectrometry and high performance liquid chromatography has revealed organic residues in (99m)Tc-pertechnetate obtained from 99Mo-molybdate by extraction using the organic solvent methylethylketone (MEK). The organic residues have been identified as either (i) low molecular weight carbonyl compounds such as formaldehyde acetaldehyde and acetone presumably caused by the effects of gamma-radiation on MEK or (ii) condensation products resulting from the action of strong alkali on MEK during the extraction process. The quantities of organic residues varied from batch to batch of extracted pertechnetate; up to 40 μg mL -1 was found. When these compounds were tested in rats by addition to a pyrophosphate bone-seeking radiopharmaceutical the tissue distribution was not significantly different from that in the control which contained no added compound. Assay for 99Tc in MEK-derived pertechnetate indicated up to 10 μg mL -1 of 99Tc carrier. An assessment of the biological effect of 99Tc carrier was obtained by (i) red blood cell labelling where 6 ng mL -1 of 99Tc was sufficient to reduce labelling efficiency; and (ii) pyrophosphate tissue distribution where a significant effect was obtained in the presence of 10 μg mL -1 of 99Tc carrier.
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Keywords
Solvent extraction, High-performance liquid chromatography, Gamma radiation, Blood cells, Residues, Carbonyls
Citation
Mohammad, R., Moore, D. E., Maddalena, D. J., & Boyd, R. E. (1984). Studies on 99mtc-pertechnetate from the MEK solvent extraction generator. (AAEC/E589). Lucas Heights, N.S.W.: Australian Atomic Energy Commission, Research Establishment.