Lipidic drug delivery systems are responsive to the human microbiome

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dc.contributor.authorCaukwell, Jen_AU
dc.contributor.authorAssenza, Sen_AU
dc.contributor.authorHassan, KAen_AU
dc.contributor.authorNeilan, BAen_AU
dc.contributor.authorClulow, AJen_AU
dc.contributor.authorManni, LSen_AU
dc.contributor.authorFong, WKen_AU
dc.date.accessioned2024-09-19T04:30:54Zen_AU
dc.date.available2024-09-19T04:30:54Zen_AU
dc.date.issued2025-01en_AU
dc.date.statistics2024-08-23en_AU
dc.description.abstractIn vitro and in vivo tests for therapeutic agents are typically conducted in sterile environments, but many target areas for drug delivery are home to thousands of microbial species. Here, we examine the behaviour of lipidic nanomaterials after exposure to representative strains of four bacterial species found in the gastrointestinal tract and skin. Small angle X-ray scattering measurements show that the nanostructure of monoolein cubic and inverse hexagonal phases are transformed, respectively, into inverse hexagonal and inverse micellar cubic phases upon exposure to a strain of live Staphylococcus aureus often present on skin and mucosa. Further investigation demonstrates that enzymatic hydrolysis and cell membrane lipid transfer are both likely responsible for this effect. The structural responses to S. aureus are rapid and significantly reduce the rate of drug release from monoolein-based nanomaterials. These findings are the first to demonstrate how a key species in the live human microbiome can trigger changes in the structure and drug release properties of lipidic nanomaterials. The effect appears to be strain specific, varies from patient to patient and body region to body region, and is anticipated to affect the bioapplication of monoglyceride-based formulations. © 2024 The Author(s). Published by Elsevier Inc - Open Access CC BY 4.0en_AU
dc.description.sponsorshipThe authors acknowledge: Jesse Cain, Daniel Nebauer, Alescia Cullen, and Joachim Larsen for scientific discussions; Frithjof Herb for his experimental assistance; Sydney Analytical for access to their SAXS instrument and Paul A. Fitzgerald for his experimental assistance; the use of facilities within the Monash X-ray Platform (MXP); the expertise and assistance of Nigel Kirby, Claire Scott, and Kirsty Brunt during time-resolved SAXS experiments conducted on the SAXS/WAXS beamline of the Australian Synchrotron, ANSTO, Australia. J.C. received support of a scholarship and funding through the ANSTO Future Now program. L.S.M. acknowledges the Swiss National Foundation of Science for financial support grant no. P2ZHP2_187769. S.A. received the support of a fellowship from “la Caixa” Foundation (ID 100010434) and from the European Union’s Horizon research and innovation programme under the Marie Skłodowska-Curie grant agreement No. 847648. The fellowship code is LCF/BQ/PI20/11760019. S.A. acknowledges support from MCIN/AEI/10.13039/501100011033 and FSE+through a Ramón y Cajal Fellowship (ref. RYC2022-037744-I).en_AU
dc.identifier.citationaukwell, J., Assenza, S., Hassan, K. A., Neilan, B. A., Clulow, A. J., Salvati Manni, L., & Fong, W.-K. (2025). Lipidic drug delivery systems are responsive to the human microbiome. Journal of Colloid and Interface Science, 677, 293-302. doi:10.1016/j.jcis.2024.07.216en_AU
dc.identifier.issn0021-9797en_AU
dc.identifier.journaltitleJournal of Colloid and Interface Scienceen_AU
dc.identifier.pagination293-302en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15684en_AU
dc.identifier.volume677en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.relation.urihttps://doi.org/10.1016/j.jcis.2024.07.216en_AU
dc.subjectLipidsen_AU
dc.subjectDrug deliveryen_AU
dc.subjectNanomaterialsen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectStaphylococcusen_AU
dc.subjectSkinen_AU
dc.subjectBodyen_AU
dc.subjectPatientsen_AU
dc.titleLipidic drug delivery systems are responsive to the human microbiomeen_AU
dc.typeJournal Articleen_AU
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