Impact of pasteurization on the self-assembly of human milk lipids during digestion

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dc.contributor.authorBinte Abu Bakar, SYen_AU
dc.contributor.authorSalim, Men_AU
dc.contributor.authorClulow, AJen_AU
dc.contributor.authorHawley, Aen_AU
dc.contributor.authorPelle, Jen_AU
dc.contributor.authorGeddes, DTen_AU
dc.contributor.authorNicholas, KRen_AU
dc.contributor.authorBoyd, BJen_AU
dc.date.accessioned2023-12-01T02:45:02Zen_AU
dc.date.available2023-12-01T02:45:02Zen_AU
dc.date.issued2022-05en_AU
dc.date.statistics2022-05-27en_AU
dc.description.abstractHuman milk is critical for the survival and development of infants. This source of nutrition contains components that protect against infections while stimulating immune maturation. In cases where the mother's own milk is unavailable, pasteurized donor milk is the preferred option. Although pasteurization has been shown to have minimal impact on the lipid and FA composition before digestion, no correlation has been made between the impact of pasteurization on the FFA composition and the self-assembly of lipids during digestion, which could act as delivery mechanisms for poorly water-soluble components. Pooled nonpasteurized and pasteurized human milk from a single donor was used in this study. The evolving FFA composition during digestion was determined using GC coupled to a flame ionization detector. In vitro digestion coupled to small-angle X-ray scattering was utilized to investigate the influence of different calcium levels, fat content, and the presence of bile salts on the extent of digestion and structural behavior of human milk lipids. Almost complete digestion was achieved when bile salts were added to the systems containing high calcium to milk fat ratio, with similar structural behavior of lipids during digestion of both types of human milk being apparent. In contrast, differences in the colloidal structures were formed during digestion in the absence of bile salt because of a greater amount of FFAs being released from the nonpasteurized than pasteurized milks. This difference in FFAs released from both types of human milk could result in varying nutritional implications for infants. © 2022 The Authors. This is an open access article under the CC BY licenceen_AU
dc.description.sponsorshipThe SAXS studies were conducted on the SAXS/WAXS beamline at the Australian Synchrotron, part of ANSTO, Victoria, Australia. The authors acknowledge the Mercy Health Breastmilk Bank for the donation of the human milk samples used in this study. This work was partly supported by the Australian Research Council.en_AU
dc.identifier.articlenumber100183en_AU
dc.identifier.citationBinte Abu Bakar, S. Y., Salim, M., Clulow, A. J., Hawley, A., Pelle, J., Geddes, D. T., Nicholas, K., R., & Boyd, B. J. (2022). Impact of pasteurization on the self-assembly of human milk lipids during digestion. Journal of Lipid Research, 63(5), 100183. doi:10.1016/j.jlr.2022.100183en_AU
dc.identifier.issn0022-2275en_AU
dc.identifier.issue5en_AU
dc.identifier.journaltitleJournal of Lipid Researchen_AU
dc.identifier.urihttps://doi.org/10.1016/j.jlr.2022.100183en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15258en_AU
dc.identifier.volume63en_AU
dc.language.isoenen_AU
dc.publisherElsevier Inc on behalf of American Society for Biochemistry and Molecular Biologyen_AU
dc.relation.urihttps://doi.org/10.1016/j.jlr.2022.100183en_AU
dc.subjectPasteurizationen_AU
dc.subjectMammary glandsen_AU
dc.subjectMilken_AU
dc.subjectLactationen_AU
dc.subjectDigestionen_AU
dc.subjectLipidsen_AU
dc.subjectNutritionen_AU
dc.subjectCalciumen_AU
dc.subjectColloidsen_AU
dc.subjectBileen_AU
dc.subjectSaltsen_AU
dc.subjectCarboxylic acidsen_AU
dc.subjectGas chromatographyen_AU
dc.subjectSmall angle scatteringen_AU
dc.titleImpact of pasteurization on the self-assembly of human milk lipids during digestionen_AU
dc.typeJournal Articleen_AU
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