Translocation of foliar absorbed Zn in sunflower (Helianthus annuus) leaves

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dc.contributor.authorLi, Cen_AU
dc.contributor.authorWang, LLen_AU
dc.contributor.authorWu, Jen_AU
dc.contributor.authorBlamey, FPCen_AU
dc.contributor.authorWang, Nen_AU
dc.contributor.authorChen, YLen_AU
dc.contributor.authorYe, Yen_AU
dc.contributor.authorWang, Len_AU
dc.contributor.authorPaterson, DJen_AU
dc.contributor.authorRead, TLen_AU
dc.contributor.authorWang, Pen_AU
dc.contributor.authorLombi, Een_AU
dc.contributor.authorWang, YHen_AU
dc.contributor.authorKopittke, PMen_AU
dc.date.accessioned2024-11-15T03:53:47Zen_AU
dc.date.available2024-11-15T03:53:47Zen_AU
dc.date.issued2022-03-02en_AU
dc.date.statistics2024-11-08en_AU
dc.description.abstractFoliar zinc (Zn) fertilization is an important approach for overcoming crop Zn deficiency, yet little is known regarding the subsequent translocation of this foliar-applied Zn. Using synchrotron-based X-ray fluorescence microscopy (XFM) and transcriptome analysis, the present study examined the translocation of foliar absorbed Zn in sunflower (Helianthus annuus) leaves. Although bulk analyses showed that there had been minimal translocation of the absorbed Zn out of the leaf within 7 days, in situ analyses showed that the distribution of Zn in the leaf had changed with time. Specifically, when Zn was applied to the leaf for 0.5 h and then removed, Zn primarily accumulated within the upper and lower epidermal layers (when examined after 3 h), but when examined after 24 h, the Zn had moved to the vascular tissues. Transcriptome analyses identified a range of genes involved in stress response, cell wall reinforcement, and binding that were initially upregulated following foliar Zn application, whereas they were downregulated after 24 h. These observations suggest that foliar Zn application caused rapid stress to the leaf, with the initial Zn accumulation in the epidermis as a detoxification strategy, but once this stress decreased, Zn was then moved to the vascular tissues. Overall, this study has shown that despite foliar Zn application causing rapid stress to the leaf and that most of the Zn stayed within the leaf over 7 days, the distribution of Zn in the leaf had changed, with Zn mostly located in the vascular tissues 24 h after the Zn had been applied. Not only do the data presented herein provide new insight for improving the efficiency of foliar Zn fertilizers, but our approach of combining XFM with a transcriptome methodological system provides a novel approach for the study of element translocation in plants. © 2022 Li, Wang, Wu, Blamey, Wang, Chen, Ye, Wang, Paterson, Read, Wang, Lombi, Wang and Kopittke. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_AU
dc.description.sponsorshipPart of the experiment was undertaken on the XFM beamline at the Australian Synchrotron, part of ANSTO. We thank the Australian Synchrotron for the beamtime and technical assistance. We also acknowledge the use of the facilities and technical assistance of the Analytical & Testing Centre of Northwestern Polytechnical University. We also would like to thank the reviewers for their valuable suggestions and careful reading of the manuscript.en_AU
dc.format.mediumElectronic-eCollectionen_AU
dc.identifier.citationLi, C., Wang, L., Wu, J., Blamey, F. P. C., Wang, N., Chen, Y., Ye, Y., Wang, L., Paterson, D. J., Read, T. L., Wang, P., Lombi, E., Wang, Y., & Kopittke, P. M. (2022). Translocation of foliar absorbed Zn in sunflower (Helianthus annuus) leaves. Frontiers in Plant Science, 13. doi:10.3389/fpls.2022.757048en_AU
dc.identifier.issn1664-462Xen_AU
dc.identifier.journaltitleFrontiers in Plant Scienceen_AU
dc.identifier.pagination757048en_AU
dc.identifier.urihttps://doi.org/10.3389/fpls.2022.757048en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15758en_AU
dc.identifier.volume13en_AU
dc.languageengen_AU
dc.language.isoenen_AU
dc.publisherFrontiersen_AU
dc.subjectSunflowersen_AU
dc.subjectPlantsen_AU
dc.subjectZincen_AU
dc.subjectSynchrotronsen_AU
dc.subjectFluorescenceen_AU
dc.subjectMicroscopyen_AU
dc.subjectTranslocationen_AU
dc.subjectGenesen_AU
dc.titleTranslocation of foliar absorbed Zn in sunflower (Helianthus annuus) leavesen_AU
dc.typeJournal Articleen_AU
dcterms.dateAccepted2022-01-17en_AU
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