Langmuir-Schaefer deposition to create an asymmetrical lipopolysaccharide sparsely tethered lipid bilayer

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dc.contributor.authorCranfield, CGen_AU
dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorGarcía, ÁLen_AU
dc.contributor.authorCornell, BAen_AU
dc.contributor.authorHolt, SAen_AU
dc.date.accessioned2025-01-08T23:39:00Zen_AU
dc.date.available2025-01-08T23:39:00Zen_AU
dc.date.issued2021-12en_AU
dc.date.statistics2024-08-28en_AU
dc.description.abstractBecause they are firmly anchored to a noble metal substrate, tethered bilayer lipid membranes (tBLMs) are considerably more robust than supported lipid bilayers such as black lipid membranes (BLMs) (Cranfield et al. Biophys J 106:182–189, 2014). The challenge to rapidly create asymmetrical tBLMs that include a lipopolysaccharide outer leaflet for bacterial model membrane research can be overcome by the use of a Langmuir-Schaefer deposition protocol. Here, we describe the procedures required to assemble and test asymmetric lipopolysaccharide (LPS) tethered lipid bilayers. © 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.en_AU
dc.format.mediumPrinten_AU
dc.identifier.booktitleMembrane Lipids: Methods and Protocolsen_AU
dc.identifier.citationCranfield, C. G., Le Brun, A. P., Garcia, A., Cornell, B. A., & Holt, S. A. (2022). Langmuir-Schaefer deposition to create an asymmetrical lipopolysaccharide sparsely tethered lipid bilayer. In C. G. Cranfield (Ed.), Membrane Lipids: Methods and Protocols, (pp. 21-30). Humana, New York : Springer. doi:10.1007/978-1-0716-1843-1_3en_AU
dc.identifier.editorsC. G. Cranfielden_AU
dc.identifier.isbn9781071618455en_AU
dc.identifier.isbn9781071618424en_AU
dc.identifier.issn1064-3745en_AU
dc.identifier.issn1940-6029en_AU
dc.identifier.pagination21-30en_AU
dc.identifier.placeofpublicationHumana, New Yorken_AU
dc.identifier.urihttps://doi.org/10.1007/978-1-0716-1843-1_3en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15855en_AU
dc.identifier.volume2402en_AU
dc.languageengen_AU
dc.language.isoenen_AU
dc.publisherSpringer Natureen_AU
dc.subjectLipidsen_AU
dc.subjectLipopolysaccharidesen_AU
dc.subjectMetalsen_AU
dc.subjectSubstratesen_AU
dc.subjectBacteriaen_AU
dc.subjectDepositionen_AU
dc.titleLangmuir-Schaefer deposition to create an asymmetrical lipopolysaccharide sparsely tethered lipid bilayeren_AU
dc.typeBook chapteren_AU
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