A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology

Simple item page
dc.contributor.authorFan, XWen_AU
dc.contributor.authorLee, KMen_AU
dc.contributor.authorJones, MWMen_AU
dc.contributor.authorHoward, DLen_AU
dc.contributor.authorCrawford, Ren_AU
dc.contributor.authorPrasadam, Ien_AU
dc.date.accessioned2023-02-20T00:54:50Zen_AU
dc.date.available2023-02-20T00:54:50Zen_AU
dc.date.issued2022-07-09en_AU
dc.date.statistics2022-09-02en_AU
dc.description.abstractThe anatomy of the osteochondral junction is complex because several tissue components exist as a unit, including uncalcified cartilage (with superficial, middle, and deep layers), calcified cartilage, and subchondral bone. Furthermore, it is difficult to study because this region is made up of a variety of cell types and extracellular matrix compositions. Using X-ray fluorescence microscopy, we present a protocol for simultaneous elemental detection on fresh frozen samples. We transferred the osteochondral sample using a tape-assisted system and successfully tested it in synchrotron X-ray fluorescence. This protocol elucidates the distinct distribution of elements at the human knee’s osteochondral junction, making it a useful tool for analyzing the co-distribution of various elements in both healthy and diseased states. © The Author(s) 2022 - Open Access under a Creative Commons Attribution 4.0 International License,en_AU
dc.description.sponsorshipI.P. would like to acknowledge The Prince Charles Hospital Research Foundation (RF-01) and the NHMRC Investigator grant fellowship (APP1176298). The authors would like to thank the CARF Histology lab in KG-Q for its contribution to the cryo, resin, and formalin-fixed paraffin-embedded sample preparation. Part of this research was undertaken on the XFM beamline at the Australian Synchrotron, part of ANSTO. Open Access funding enabled and organized by CAUL and its Member Institutions. National Health and Medical Research Council, APP1176298, Prince Charles Hospital Research Foundation, RF-01.en_AU
dc.identifier.citationFan, X., Lee, K. M., Jones, M. W. M., Howard, D., Crawford, R., & Prasadam, I. (2022). A technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiology. Histochemistry and Cell Biology, 158, 463–469. doi:10.1007/s00418-022-02135-8en_AU
dc.identifier.issn1432-119Xen_AU
dc.identifier.journaltitleHistochemistry and Cell Biologyen_AU
dc.identifier.pagination463-469en_AU
dc.identifier.urihttps://doi.org/10.1007/s00418-022-02135-8en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14671en_AU
dc.identifier.volume158en_AU
dc.language.isoenen_AU
dc.publisherSpringer Natureen_AU
dc.subjectCartilageen_AU
dc.subjectBone jointsen_AU
dc.subjectConnective tissueen_AU
dc.subjectBone tissuesen_AU
dc.subjectElementsen_AU
dc.subjectTrace amountsen_AU
dc.subjectZincen_AU
dc.subjectLeaden_AU
dc.subjectCalciumen_AU
dc.subjectStrontiumen_AU
dc.subjectSynchrotronsen_AU
dc.subjectEtiologyen_AU
dc.subjectDiseasesen_AU
dc.titleA technique for preparing undecalcified osteochondral fresh frozen sections for elemental mapping and understanding disease etiologyen_AU
dc.typeJournal Articleen_AU
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
s00418-022-02135-8.pdf
Size:
3.59 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.63 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Journal Articles