Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques

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dc.contributor.authorRussell, RAen_AU
dc.contributor.authorGarvey, CJen_AU
dc.contributor.authorDarwish, TAen_AU
dc.contributor.authorFoster, LJRen_AU
dc.contributor.authorHolden, PJen_AU
dc.date.accessioned2024-02-22T22:24:46Zen_AU
dc.date.available2024-02-22T22:24:46Zen_AU
dc.date.issued2015en_AU
dc.date.statistics2024-02-09en_AU
dc.description.abstractThe use of microbial biosynthesis to produced deuterated recombinant proteins is a well-established practice in investigations of the relationship between molecular structure and function using neutron scattering and nuclear magnetic resonance spectroscopy. However, there have been few reports of using microbial synthetic capacity to produce labeled native biopolymers. Here, we describe methods for the production of deuterated polyhydroxyalkanoate biopolyesters in bacteria, the polysaccharide chitosan in the yeast Pichia pastoris, and cellulose in the bacterium Gluconacetobacter xylinus. The resulting molecules offer not only multiple options in creating structural contrast in polymer blends and composites in structural studies but also insight into the biosynthetic pathways themselves. © 2015 Elsevier Inc.en_AU
dc.identifier.booktitleIsotope labeling of biomolecules - labeling methodsen_AU
dc.identifier.chapter5en_AU
dc.identifier.citationRussell, R. A., Garvey, C. J., Darwish, T. A., Foster, L. J. R., & Holden, P. J. (2015). Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques. In Z. Kelman (Ed), Isotope labeling of biomolecules - labeling methods, Methods in enzymology, (Vol. 565, pp. 97-121). Amsterdam: Academic Press. doi:10.1016/bs.mie.2015.06.015en_AU
dc.identifier.editorsZ. Kelmanen_AU
dc.identifier.isbn978-0-12-803048-6en_AU
dc.identifier.issn0076-6879en_AU
dc.identifier.pagination97-121en_AU
dc.identifier.placeofpublicationAmsterdamen_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15393en_AU
dc.identifier.volume565en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.relation.ispartofseriesMethods in enzymologyen_AU
dc.relation.urihttps://doi.org/10.1016/bs.mie.2015.06.015en_AU
dc.subjectPolymersen_AU
dc.subjectDeuterationen_AU
dc.subjectNeutron diffractionen_AU
dc.subjectBiosynthesisen_AU
dc.subjectProteinsen_AU
dc.subjectMolecular structureen_AU
dc.subjectNMR Spectrometersen_AU
dc.titleBiopolymer deuteration for neutron scattering and other isotope-sensitive techniquesen_AU
dc.typeBook chapteren_AU
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