Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
The use of microbial biosynthesis to produced deuterated recombinant proteins is a well-established practice in investigations of the relationship between molecular structure and function using neutron scattering and nuclear magnetic resonance spectroscopy. However, there have been few reports of using microbial synthetic capacity to produce labeled native biopolymers. Here, we describe methods for the production of deuterated polyhydroxyalkanoate biopolyesters in bacteria, the polysaccharide chitosan in the yeast Pichia pastoris, and cellulose in the bacterium Gluconacetobacter xylinus. The resulting molecules offer not only multiple options in creating structural contrast in polymer blends and composites in structural studies but also insight into the biosynthetic pathways themselves. © 2015 Elsevier Inc.
Description
Keywords
Polymers, Deuteration, Neutron diffraction, Biosynthesis, Proteins, Molecular structure, NMR Spectrometers
Citation
Russell, R. A., Garvey, C. J., Darwish, T. A., Foster, L. J. R., & Holden, P. J. (2015). Biopolymer deuteration for neutron scattering and other isotope-sensitive techniques. In Z. Kelman (Ed), Isotope labeling of biomolecules - labeling methods, Methods in enzymology, (Vol. 565, pp. 97-121). Amsterdam: Academic Press. doi:10.1016/bs.mie.2015.06.015