Effect of silicon on the distribution and speciation of uranium in sunflower (Helianthus annuus)

Sunflower (Helianthus annuus) can potentially be used for uranium (U) phytoremediation. However, the factors influencing the absorption of U and its subsequent distribution within plant tissues remain unclear, including the effect of silicon (Si) which is known to increase metal tolerance. Here, using hydroponics, the effect of Si on the distribution and speciation of U in sunflower was examined using synchrotron-based X–ray fluorescence and fluorescence-X-ray absorption near-edge spectroscopy. It was found that ∼88 % of U accumulates within the root regardless of treatments. Without the addition of Si, most of the U appeared to bind to epidermis within the roots, whereas in the leaves, U primarily accumulated in the veins. The addition of Si alleviated U phytotoxicity and decreased U concentration in sunflower by an average of 60 %. In the roots, Si enhanced U distribution in cell walls and impeded its entry into cells, likely due to increased callose deposition. In the leaves, Si induced the sequestration of U in trichomes. However, Si did not alter U speciation and U remained in the hexavalent form. These results provide information on U accumulation and distribution within sunflower, and suggest that Si could enhance plant growth under high U stress. © 2024 Elsevier B.V.

Keywords

Uranium, Silicon, Sunflowers, Distribution, Plant tissues, Synchrotrons, X-ray fluorescence analysis, Absorption, Toxicity, Plants, Spectroscopy

Citation

Wang, L., Liang, Y., Liu, S., Chen, F., Ye, Y., Chen, Y., Wang, J., Paterson, D. J., Kopittke, P. M., Wang, Y., & Li, C. (2024). Effect of silicon on the distribution and speciation of uranium in sunflower (Helianthus annuus). Journal of Hazardous Materials, 478, 135433. doi:10.1016/j.jhazmat.2024.135433