Test-retest reliability and inter scanner variability of 11C-raclopride striatal binding potentials between two INVEON PET/CT imaging systems for naïve Sprague Dawley rats
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Date
2014-04-16
Journal Title
Journal ISSN
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Publisher
Wiley
Abstract
Background: 11C-raclopride is a routine tracer for quantification of dopamine D2 receptors in neurological and psychiatric disease. D2 imaging in key longitudinal models has significant utility of understanding mechanisms and therapeutic interventions.
Aims: Optimisation of preclinical imaging and data analysis protocols for 11C-raclopride in rat brain.
Methods:
a) Test-retest reliability: Naïve male Sprague Dawley rats (n = 6) underwent test-retest assessment of binding potential variability, with two scans, 1 week apart. Rats were anaesthetised (1–5% isoflurane) and received 11C-raclopride (>0.1 nmol, 20–40 MBq) during 1 hour image acquisition (Siemens Inveon PET/CT), followed by a 10 minute CT scan.
b) Assessment of the intersystem variability of the INVEON scanners (n = 12). Test-retest experiments were performed on a second INVEON system.
c) Assessment of inter system variability with arterial blood sampling (n = 5). Acquisitions were performed (as above) with prior femoral artery cannulation: 23 blood samples (∼30 ul) were collected during PET acquisition, and plasma metabolite corrected input functions generated.
PET list mode data were histogrammed (23 frames) and reconstructed with 2D filtered backprojection algorithm. The impact of some post-reconstruction image processing techniques, such iterative deconvolution of the image and data denoising techniques, onto the accuracy and reliability of the computed parameter of interest were also investigated. Binding potential parametric maps were calculated from the dynamic PET data (using either a standard reference tissue modelling using the cerebellum TAC (test-retest), and or a 2 compartment kinetic modelling with input function).
Preliminary results: Significant improvements were seen for tissue activity data after denoising /iterative deconvolution (see figure). Analysis of binding potential data are currently in progress.
Conclusion: Assessment of within and intersystem variability will aid the appropriate statistical design of future longitudinal 11C-raclopride imaging studies. Improvements from post-reconstruction image processing techniques show significant benefits. © 1999-2022 John Wiley & Sons, Inc.
Description
Keywords
Positron computed tomography, Image scanners, Rats, Dopamine, Receptors, Data, Experiment results, Simulation
Citation
Callaghan, P. D., Zahra, D., Wimberley, C. A., Arthur, A., Rahardjo, G. L., Hamze, H., Davis, E., Nguyen, A., Boisson, F., Perkins, G., Pascali, G., Reilhac,A.. & Gregoire. M. C. (2014). Test-retest reliability and inter scanner variability of 11C-raclopride striatal binding potentials between two INVEON PET/CT imaging systems for naïve Sprague Dawley rats. Oral presentation to the 44th ANSSNM 2014 Conference, 25- 28 April, 2014 Adelaide, Australia. In Internal Medicine Journal, 44(S1), 1-18. doi:10.1111/imj.12418