Please use this identifier to cite or link to this item: https://apo.ansto.gov.au/dspace/handle/10238/5483
Title: Correlation of thermostability and conformational changes of catechol 2, 3-dioxygenases from two disparate micro-organisms
Authors: Sokolova, AV
Huang, SL
Duff, AP
Gilbert, EP
Li, WH
Keywords: Scattering
X-ray lasers
Crystal structure
Crystals
Purification
Decomposition
Issue Date: 1-Oct-2013
Publisher: Elsevier Science BV
Citation: Sokolova, A., Huang, S. L., Duff, A., Gilbert, E. P., & Li, W. H. (2013). Correlation of thermostability and conformational changes of catechol 2, 3-dioxygenases from two disparate micro-organisms. Biophysical Chemistry, 180, 145-152. doi:10.1016/j.bpc.2013.07.012
Abstract: We have investigated the structure of recombinant catechol 2, 3-dioxygenase (C23O) purified from two species in which the enzyme has evolved to function at different temperature. The two species are mesophilic bacterium Pseudomonas putida strain mt-2 and thermophilic archaea Sulfolobus acidocaldarius DSM639. Using the primary sequence analysis, we show that both C23Os have only 30% identity and 48% similarity but contain conserved amino acid residues forming an active site area around the iron ion. The corresponding differences in homology, but structural similarity in active area residues, appear to provide completely different responses to heating the two enzymes. We confirm this by small angle X-ray scattering and demonstrate that the overall structure of C23O from P. putida is slightly different from its crystalline form whereas the solution scattering of C230 from S. acidocaldarius at temperatures between 4 and 85 degrees C ideally fits the calculated scattering from the single crystal structure. The thermostability of C230 from S. acidocaldarius correlates well with conformation in solution during thermal treatment. The similarity of the two enzymes in primary and tertiary structure may be taken as a confirmation that two enzymes have evolved from a common ancestor. © 2013, Elsevier Ltd.
Gov't Doc #: 5416
URI: http://dx.doi.org/10.1016/j.bpc.2013.07.012
http://apo.ansto.gov.au/dspace/handle/10238/5483
ISSN: 0301-4622
Appears in Collections:Journal Articles

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