Correlation of thermostability and conformational changes of catechol 2, 3-dioxygenases from two disparate micro-organisms

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dc.contributor.authorSokolova, AVen_AU
dc.contributor.authorHuang, SLen_AU
dc.contributor.authorDuff, APen_AU
dc.contributor.authorGilbert, EPen_AU
dc.contributor.authorLi, WHen_AU
dc.date.accessioned2014-04-24T04:19:19Zen_AU
dc.date.available2014-04-24T04:19:19Zen_AU
dc.date.issued2013-10-01en_AU
dc.date.statistics2014-04-24en_AU
dc.description.abstractWe have investigated the structure of recombinant catechol 2, 3-dioxygenase (C23O) purified from two species in which the enzyme has evolved to function at different temperature. The two species are mesophilic bacterium Pseudomonas putida strain mt-2 and thermophilic archaea Sulfolobus acidocaldarius DSM639. Using the primary sequence analysis, we show that both C23Os have only 30% identity and 48% similarity but contain conserved amino acid residues forming an active site area around the iron ion. The corresponding differences in homology, but structural similarity in active area residues, appear to provide completely different responses to heating the two enzymes. We confirm this by small angle X-ray scattering and demonstrate that the overall structure of C23O from P. putida is slightly different from its crystalline form whereas the solution scattering of C230 from S. acidocaldarius at temperatures between 4 and 85 degrees C ideally fits the calculated scattering from the single crystal structure. The thermostability of C230 from S. acidocaldarius correlates well with conformation in solution during thermal treatment. The similarity of the two enzymes in primary and tertiary structure may be taken as a confirmation that two enzymes have evolved from a common ancestor. © 2013, Elsevier Ltd.en_AU
dc.identifier.citationSokolova, A., Huang, S. L., Duff, A., Gilbert, E. P., & Li, W. H. (2013). Correlation of thermostability and conformational changes of catechol 2, 3-dioxygenases from two disparate micro-organisms. Biophysical Chemistry, 180, 145-152. doi:10.1016/j.bpc.2013.07.012en_AU
dc.identifier.govdoc5416en_AU
dc.identifier.issn0301-4622en_AU
dc.identifier.journaltitleBiophysical Chemistryen_AU
dc.identifier.pagination145-152en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.bpc.2013.07.012en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5483en_AU
dc.identifier.volume180en_AU
dc.language.isoenen_AU
dc.publisherElsevier Science BVen_AU
dc.subjectScatteringen_AU
dc.subjectX-ray lasersen_AU
dc.subjectCrystal structureen_AU
dc.subjectCrystalsen_AU
dc.subjectPurificationen_AU
dc.subjectDecompositionen_AU
dc.titleCorrelation of thermostability and conformational changes of catechol 2, 3-dioxygenases from two disparate micro-organismsen_AU
dc.typeJournal Articleen_AU
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