Please use this identifier to cite or link to this item: https://apo.ansto.gov.au/dspace/handle/10238/5241
Title: The antimicrobial peptide aurein 1.2 disrupts model membranes via the carpet mechanism
Authors: Fernandez, DI
Le Brun, AP
Whitwell, TC
Sani, MA
James, M
Separovic, F
Keywords: Microscopy
Cell membranes
Phospholipids
Microbalances
Reflectivity
Magnetic resonance
Issue Date: 1-Jan-2012
Publisher: Royal Society of Chemistry
Citation: Fernandez, D. I., Le Brun, A. P., Whitwell, T. C., Sani, M. A., James, M., & Separovic, F. (2012). The antimicrobial peptide aurein 1.2 disrupts model membranes via the carpet mechanism. Physical Chemistry Chemical Physics, 14(45), 15739-15751. doi:10.1039/c2cp43099a
Abstract: The membrane interactions of the antimicrobial peptide aurein 1.2 were studied using a range of biophysical techniques to determine the location and the mechanism of action in DMPC (dimyristoylphosphatidylcholine) and DMPC/DMPG (dimyristoylphosphatidylglycerol) model membranes that mimic characteristics of eukaryotic and prokaryotic membranes, respectively. Neutron reflectometry and solid-state NMR revealed subtle changes in membrane structure caused by the peptide. Quartz crystal microbalance with dissipation, vesicle dye leakage and atomic force microscopy measurements were used to investigate the global mode of peptide interaction. Aurein 1.2 displayed an enhanced interaction with the anionic DMPC/DMPG membrane while exhibiting primarily a surface interaction with both types of model membranes, which led to bilayer disruption and membrane lysis. The antimicrobial peptide interaction is consistent with the carpet mechanism for aurein 1.2 with discrete structural changes depending on the type of phospholipid membrane. © 2012, Royal Society of Chemistry
Gov't Doc #: 4567
URI: http://dx.doi.org/10.1039/c2cp43099a
http://apo.ansto.gov.au/dspace/handle/10238/5241
ISSN: 1463-9076
Appears in Collections:Journal Articles

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