The antimicrobial peptide aurein 1.2 disrupts model membranes via the carpet mechanism

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dc.contributor.authorFernandez, DIen_AU
dc.contributor.authorLe Brun, APen_AU
dc.contributor.authorWhitwell, TCen_AU
dc.contributor.authorSani, MAen_AU
dc.contributor.authorJames, Men_AU
dc.contributor.authorSeparovic, Fen_AU
dc.date.accessioned2014-03-05T03:18:25Zen_AU
dc.date.available2014-03-05T03:18:25Zen_AU
dc.date.issued2012-01-01en_AU
dc.date.statistics2014-03-05en_AU
dc.description.abstractThe membrane interactions of the antimicrobial peptide aurein 1.2 were studied using a range of biophysical techniques to determine the location and the mechanism of action in DMPC (dimyristoylphosphatidylcholine) and DMPC/DMPG (dimyristoylphosphatidylglycerol) model membranes that mimic characteristics of eukaryotic and prokaryotic membranes, respectively. Neutron reflectometry and solid-state NMR revealed subtle changes in membrane structure caused by the peptide. Quartz crystal microbalance with dissipation, vesicle dye leakage and atomic force microscopy measurements were used to investigate the global mode of peptide interaction. Aurein 1.2 displayed an enhanced interaction with the anionic DMPC/DMPG membrane while exhibiting primarily a surface interaction with both types of model membranes, which led to bilayer disruption and membrane lysis. The antimicrobial peptide interaction is consistent with the carpet mechanism for aurein 1.2 with discrete structural changes depending on the type of phospholipid membrane. © 2012, Royal Society of Chemistryen_AU
dc.identifier.citationFernandez, D. I., Le Brun, A. P., Whitwell, T. C., Sani, M. A., James, M., & Separovic, F. (2012). The antimicrobial peptide aurein 1.2 disrupts model membranes via the carpet mechanism. Physical Chemistry Chemical Physics, 14(45), 15739-15751. doi:10.1039/c2cp43099aen_AU
dc.identifier.govdoc4567en_AU
dc.identifier.issn1463-9076en_AU
dc.identifier.issue45en_AU
dc.identifier.journaltitlePhysical Chemistry Chemical Physicsen_AU
dc.identifier.pagination15739-15751en_AU
dc.identifier.urihttp://dx.doi.org/10.1039/c2cp43099aen_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/5241en_AU
dc.identifier.volume14en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectMicroscopyen_AU
dc.subjectCell membranesen_AU
dc.subjectPhospholipidsen_AU
dc.subjectMicrobalancesen_AU
dc.subjectReflectivityen_AU
dc.subjectMagnetic resonanceen_AU
dc.titleThe antimicrobial peptide aurein 1.2 disrupts model membranes via the carpet mechanismen_AU
dc.typeJournal Articleen_AU
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