Please use this identifier to cite or link to this item: https://apo.ansto.gov.au/dspace/handle/10238/4533
Title: Structural effects of the antimicrobial peptide maculatin 1.1 on supported lipid bilayers
Authors: Fernandez, DI
Brun, AP
Lee, TH
Bansal, PS
Keywords: Membranes
Peptides
Polarization
Interferometry
Neutron reflectors
Orientation
Issue Date: 1-Jan-2013
Publisher: Springer
Citation: Fernandez, D.I., Brun, A.P., Lee, T. H., & Bansal, P.(2013). European Biophysics Journal with Biophysics Letters, 42(1), 47-59. doi:10.1007/s00249-012-0796-6
Abstract: The interactions of the antimicrobial peptide maculatin 1.1 (GLFGVLAKVAAHVVPAIAEHF-NH2) with model phospholipid membranes were studied by use of dual polarisation interferometry and neutron reflectometry and dimyristoylphosphatidylcholine (DMPC) and mixed DMPC–dimyristoylphosphatidylglycerol (DMPG)-supported lipid bilayers chosen to mimic eukaryotic and prokaryotic membranes, respectively. In DMPC bilayers concentration-dependent binding and increasing perturbation of bilayer order by maculatin were observed. By contrast, in mixed DMPC–DMPG bilayers, maculatin interacted more strongly and in a concentration-dependent manner with retention of bilayer lipid order and structure, consistent with pore formation. These results emphasise the importance of membrane charge in mediating antimicrobial peptide activity and emphasise the importance of using complementary methods of analysis in probing the mode of action of antimicrobial peptides.© 2013, Springer.
Gov't Doc #: 4907
URI: http://dx.doi.org/10.1007/s00249-012-0796-6
http://apo.ansto.gov.au/dspace/handle/10238/4533
ISSN: 0175-7571
Appears in Collections:Journal Articles

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