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Title: Lipopolysaccharide-stimulated interleukin-10 release from neonatal spinal cord microglia is potentiated by glutamate.
Authors: Werry, EL
Liu, GJ
Lovelace, MD
Nagarajah, R
Hickie, IB
Bennett, MR
Keywords: Lipopolysaccharides
Spinal cord
Central nervous system
Enzyme immunoassay
Issue Date: 17-Feb-2011
Publisher: Elsevier
Citation: Werry, E. L., Liu, G. J., Lovelace, M. D., Nagarajah, R., Hichie, I. B., & Bennett, M. R. (2011). Lipopolysaccharide-stimulated interleukin-10 release from neonatal spinal cord microglia is potentiated by glutamate. Neuroscience, 175, 93-103. doi:10.1016/j.neuroscience.2010.10.080
Abstract: Interleukin-10 (IL-10) is a cytokine with important endogenous and therapeutic anti-inflammatory effects. Given this, it is of interest to investigate factors that modulate IL-10 levels in the central nervous system. IL-10 is released after lipopolysaccharide (LPS) stimulation of microglia. Microglia also express functional glutamate receptors and in inflammatory conditions are exposed to increased levels of glutamate. The aim of this research, then, is to investigate whether glutamate can modulate lipopolysaccharide stimulation of IL-10 release from neonatal rat spinal cord microglia. Enzyme-linked immunosorbent assays (ELISAs) were used to quantify IL-10 release from cultured neonatal spinal cord microglia and reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure IL-10 mRNA expression. Glutamate (1 mM) significantly increased LPS (1 μg/ml)-stimulated IL-10 release from microglia by 172% (EC50 of 103 μM) and significantly upregulated IL-10 mRNA levels. Glutamate potentiated LPS-stimulated IL-10 release by binding all subtypes of glutamate receptor. These results show that glutamate substantially increases the release of an anti-inflammatory cytokine from neonatal spinal cord microglia activated by a high concentration of LPS. © 2011, Elsevier Ltd.
Gov't Doc #: 3316
ISSN: 0306-4522
Appears in Collections:Journal Articles

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