Lipopolysaccharide-stimulated interleukin-10 release from neonatal spinal cord microglia is potentiated by glutamate

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dc.contributor.authorWerry, ELen_AU
dc.contributor.authorLiu, GJen_AU
dc.contributor.authorLovelace, MDen_AU
dc.contributor.authorNagarajah, Ren_AU
dc.contributor.authorHickie, IBen_AU
dc.contributor.authorBennett, MRen_AU
dc.date.accessioned2011-03-18T00:57:22Zen_AU
dc.date.available2011-03-18T00:57:22Zen_AU
dc.date.issued2011-02-17en_AU
dc.date.statistics2011-02-17en_AU
dc.description.abstractInterleukin-10 (IL-10) is a cytokine with important endogenous and therapeutic anti-inflammatory effects. Given this, it is of interest to investigate factors that modulate IL-10 levels in the central nervous system. IL-10 is released after lipopolysaccharide (LPS) stimulation of microglia. Microglia also express functional glutamate receptors and in inflammatory conditions are exposed to increased levels of glutamate. The aim of this research, then, is to investigate whether glutamate can modulate lipopolysaccharide stimulation of IL-10 release from neonatal rat spinal cord microglia. Enzyme-linked immunosorbent assays (ELISAs) were used to quantify IL-10 release from cultured neonatal spinal cord microglia and reverse transcriptase-polymerase chain reaction (RT-PCR) was used to measure IL-10 mRNA expression. Glutamate (1 mM) significantly increased LPS (1 μg/ml)-stimulated IL-10 release from microglia by 172% (EC50 of 103 μM) and significantly upregulated IL-10 mRNA levels. Glutamate potentiated LPS-stimulated IL-10 release by binding all subtypes of glutamate receptor. These results show that glutamate substantially increases the release of an anti-inflammatory cytokine from neonatal spinal cord microglia activated by a high concentration of LPS. © 2011, Elsevier Ltd.en_AU
dc.identifier.citationWerry, E. L., Liu, G. J., Lovelace, M. D., Nagarajah, R., Hichie, I. B., & Bennett, M. R. (2011). Lipopolysaccharide-stimulated interleukin-10 release from neonatal spinal cord microglia is potentiated by glutamate. Neuroscience, 175, 93-103. doi:10.1016/j.neuroscience.2010.10.080en_AU
dc.identifier.govdoc3316en_AU
dc.identifier.issn0306-4522en_AU
dc.identifier.journaltitleNeuroscienceen_AU
dc.identifier.pagination93-103en_AU
dc.identifier.urihttp://dx.doi.org/10.1016/j.neuroscience.2010.10.080en_AU
dc.identifier.urihttp://apo.ansto.gov.au/dspace/handle/10238/3143en_AU
dc.identifier.volume175en_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectLipopolysaccharidesen_AU
dc.subjectSpinal corden_AU
dc.subjectInflammationen_AU
dc.subjectLymphokinesen_AU
dc.subjectCentral nervous systemen_AU
dc.subjectEnzyme immunoassayen_AU
dc.titleLipopolysaccharide-stimulated interleukin-10 release from neonatal spinal cord microglia is potentiated by glutamateen_AU
dc.typeJournal Articleen_AU
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