Tuning the hierarchical structure and resilience of resilin-like polypeptide hydrogels using graphene oxide

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dc.contributor.authorBalu, Ren_AU
dc.contributor.authorDorishetty, Pen_AU
dc.contributor.authorMata, JPen_AU
dc.contributor.authorHill, AJen_AU
dc.contributor.authorDutta, NKen_AU
dc.contributor.authorChoudhury, NRen_AU
dc.date.accessioned2023-11-07T04:47:59Zen_AU
dc.date.available2023-11-07T04:47:59Zen_AU
dc.date.issued2020-11-24en_AU
dc.date.statistics2023-11-07en_AU
dc.description.abstractResilin-like polypeptides (RLPs) are an important class of intrinsically disordered multistimuli-responsive bioelastomers. The nanostructure of RLPs in solution has been extensively studied in the past few years, from dilute to molecular crowding conditions, and with the addition of rigid biopolymers. Modification of the hierarchical network structure of RLP hydrogels using graphene oxide (GO) as an additive is a burgeoning prospect for their application in the bioelectronic and biomedical fields. In this work, we systemically study the influence of incorporating GO into RLP (Rec1) hydrogels for tuning their physicochemical properties and understanding the gel–cell interactions. The nature of GO interaction with the Rec1 hydrogel is deduced from the change in structure and properties. Contrast-matching small-angle and ultra-small-angle neutron-scattering techniques were used to investigate the network structure of the Rec1 hydrogel and how this structure is modified in the presence of GO. Incorporation of GO in the Rec1 hydrogel matrix results in an increase in the micromechanical resilience, equilibrium water swelling ratio, micropore size, cross-linked domain size; with a decrease in the cross-link density, mass fractal cluster size, local compressive elastic modulus, and cell inert characteristics. These property combinations achieved with the addition of GO further open up the available structure–property design window for RLP applications. © 2020 American Chemical Societyen_AU
dc.description.sponsorshipThis research was supported by Australian Research Council’s Discovery Projects funding scheme (project DP160101267). The authors acknowledge the facilities and the scientific and technical assistance of the RMIT Microscopy & Microanalysis Research Facility (especially, Dr. Chaitali Dekiwadia for Bio-EM data analysis), RMIT Micro Nano Research Facility, and BioFab3D of St. Vincent’s Hospital (Melbourne). SANS and USANS experiments at ACNS were supported by ANSTO’s beamtime award (P6656). This work benefited from the use of free SasView application.en_AU
dc.identifier.citationBalu, R., Dorishetty, P., Mata, J. P., Hill, A. J., Dutta, N. K., & Choudhury, N. R. (2020). Tuning the hierarchical structure and resilience of resilin-like polypeptide hydrogels using graphene oxide. ACS Applied Bio Materials, 3(12), 8688-8697. doi:10.1021/acsabm.0c01088en_AU
dc.identifier.issn2576-6422en_AU
dc.identifier.issue12en_AU
dc.identifier.journaltitleACS Applied Bio Materialsen_AU
dc.identifier.pagination8688-8697en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/15172en_AU
dc.identifier.volume3en_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Societyen_AU
dc.relation.urihttps://doi.org/10.1021/acsabm.0c01088en_AU
dc.subjectGrapheneen_AU
dc.subjectOxidesen_AU
dc.subjectHydrogelsen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectCell culturesen_AU
dc.subjectPolypeptidesen_AU
dc.subjectNanostructuresen_AU
dc.titleTuning the hierarchical structure and resilience of resilin-like polypeptide hydrogels using graphene oxideen_AU
dc.typeJournal Articleen_AU
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