Dose-on-demand production of diverse 18F-radiotracers for preclinical applications using a continuous flow microfluidic system

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dc.contributor.authorMatesic, Len_AU
dc.contributor.authorKallinen, Aen_AU
dc.contributor.authorGreguric, IDen_AU
dc.contributor.authorPascali, Gen_AU
dc.date.accessioned2025-04-03T05:09:23Zen_AU
dc.date.available2025-04-03T05:09:23Zen_AU
dc.date.issued2017-09en_AU
dc.date.statistics2025-03-11en_AU
dc.description.abstractThe production of 18F-radiotracers using continuous flow microfluidics is under-utilized due to perceived equipment limitations. We describe the dose-on-demand principle, whereby the back-to-back production of multiple, diverse 18F-radiotracers can be prepared on the same day, on the same microfluidic system using the same batch of [18F]fluoride, the same microreactor, the same HPLC column and SPE cartridge to obtain a useful production yield. [18F]MEL050, [18F]Fallypride and [18F]PBR111 were radiolabeled with [18F]fluoride using the Advion NanoTek Microfluidic Synthesis System. The outlet of the microreactor was connected to an automated HPLC injector and following the collection of the product, SPE reformulation produced the 18F-radiotracer in <10% ethanolic saline. A thorough automated cleaning procedure was implemented to ensure no cross-contamination between radiotracer synthesis. The complete productions for [18F]MEL050 and [18F]Fallypride were performed at total flow rates of 20 μL/min, resulting in 40 ± 13% and 25 ± 13% RCY respectively. [18F]PBR111 was performed at 200 μL/min to obtain 27 ± 8% RCY. Molar activities for each 18F-radiotracer were >100 GBq/μmol and radiochemical purities were >97%, implying that the cleaning procedure was effective. Using the same initial solution of [18F]fluoride, microreactor, HPLC column and SPE cartridge, three diverse 18F-radiotracers could be produced in yields sufficient for preclinical studies in a back-to-back fashion using a microfluidic system with no detectable cross-contamination. Crown Copyright © 2017 Published by Elsevier Inc. All rights reserved.en_AU
dc.format.mediumPrint-Electronicen_AU
dc.identifier.citationMatesic, L., Kallinen, A., Greguric, I., & Pascali, G. (2017). Dose-on-demand production of diverse 18F-radiotracers for preclinical applications using a continuous flow microfluidic system. Nuclear Medicine and Biology, 52, 24-31. doi:10.1016/j.nucmedbio.2017.05.004en_AU
dc.identifier.issn0969-8051en_AU
dc.identifier.issn1872-9614en_AU
dc.identifier.journaltitleNuclear Medicine and Biologyen_AU
dc.identifier.pagination24-31en_AU
dc.identifier.urihttps://doi.org/10.1016/j.nucmedbio.2017.05.004en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/16114en_AU
dc.identifier.volume52en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherElsevieren_AU
dc.subjectRadiopharmaceuticalsen_AU
dc.subjectTracer techniquesen_AU
dc.subjectFluorine 18en_AU
dc.subjectProductionen_AU
dc.subjectPositron computed tomographyen_AU
dc.subjectFluidic Devicesen_AU
dc.titleDose-on-demand production of diverse 18F-radiotracers for preclinical applications using a continuous flow microfluidic systemen_AU
dc.typeJournal Articleen_AU
dcterms.dateAccepted2017-05-10en_AU
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