Morphological changes in melanoma cells following irradiation

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dc.contributor.authorBarkla, DHen_AU
dc.contributor.authorAllen, BJen_AU
dc.contributor.authorBrown, JKen_AU
dc.contributor.authorBuck, Sen_AU
dc.date.accessioned2025-11-26T01:31:43Zen_AU
dc.date.available2025-11-26T01:31:43Zen_AU
dc.date.issued1989-09-22en_AU
dc.date.statistics2025-11-12en_AU
dc.descriptionPhysical copy held by ANSTO Library at DDC: 571.45/14en_AU
dc.description.abstractThree lines of melanoma cells (two human, one murine) were propagated in tissue culture flasks and then subjected to either gamma irradiation (using a 60Co, source) or thermal neutron irradiation (in the MOATA reactor). Some flasks were treated with 10 boronophenylalanine (BPA) prior to neutron irradiation to simulate neutron capture therapy (NCT) . Flasks of cells were then fixed at 24 hr intervals over the next 20 days and prepared for examination using light microscopy and transmission electron microscopy. Other flasks of cells were analysed for melanin content before and after irradiation. During days 1-5 after irradiation many cells detached from the floor of the flasks and died. Changes in cell morphology after irradiation were similar for gamma-treated and 10B +ve and 10B -ve NCT-treated cells. Early changes seen during days 1-3 included abnormalities in both the plasma membrane and the nuclear membrane of melanoma cells, changes presumably leading to cell death. During days 6-12, surviving cells showed evidence of differentiation into subpopulations of cells showing increased melanin content . During days 13-20, these changes continued and, in addition, flasks were gradually repopulated with cells having a morphology similar to that of the pre-irradiated cells . The apparent increase in melanin content after irradiation was confirmed statistically using a melanin assay. The results suggest that gamma and thermal neutron irradiation operate similarly, and disrupt the integrity of both nuclear and plasma membranes. The relationship between these changes and damage to DNA is unclear. Morphological changes distinctive of the neutron capture reaction of thermal neutrons by 10BPA were not apparent. © The Author.en_AU
dc.identifier.booktitle12th AINSE Radiation Biology Conference, 22-24th September 1989, Lucas Heights - AINSE Theatre : conference handbook (programme, abstracts and general information)en_AU
dc.identifier.citationBarkla, D. H., Allen, B. J., Brown, J. K., & Buck, S. (1989). Morphological changes in melanoma cells following irradiation. Presentation to the 12th AINSE Radiation Biology Conference, 22-24th September 1989, Lucas Heights, (pp. 20). Lucas Heights, New South Wales : AINSE.en_AU
dc.identifier.conferenceenddate1989-09-24en_AU
dc.identifier.conferencename12th AINSE Radiation Biology Conferenceen_AU
dc.identifier.conferenceplaceLucas Heights, New South Walesen_AU
dc.identifier.conferencestartdate1989-09-22en_AU
dc.identifier.pagination20en_AU
dc.identifier.placeofpublicationLucas Heights, New South Walesen_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/16740en_AU
dc.language.isoenen_AU
dc.publisherAINSEen_AU
dc.relation.ispartofseries1en_AU
dc.subjectAnimal cellsen_AU
dc.subjectBoron 10en_AU
dc.subjectCell killingen_AU
dc.subjectCobalt 60en_AU
dc.subjectGamma radiationen_AU
dc.subjectIrradiationen_AU
dc.subjectMelaninen_AU
dc.subjectNeutron capture therapyen_AU
dc.subjectTumor cellsen_AU
dc.subjectTherapyen_AU
dc.titleMorphological changes in melanoma cells following irradiationen_AU
dc.typeConference Abstracten_AU
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