Direct imaging of endogenous biometal distributions within millimetre-scale organisms at micrometre resolution – x-ray fluorescence tomography

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dc.contributor.authorde Jonge, MDen_AU
dc.contributor.authorRuben, Gen_AU
dc.contributor.authorMayo, SSen_AU
dc.contributor.authorRyan, CGen_AU
dc.contributor.authorKirkham, Ren_AU
dc.contributor.authorHoward, DLen_AU
dc.contributor.authorPaterson, DJen_AU
dc.date.accessioned2023-01-27T01:00:40Zen_AU
dc.date.available2023-01-27T01:00:40Zen_AU
dc.date.issued2016-02-04en_AU
dc.date.statistics2022-11-04en_AU
dc.description.abstractFirst-row transition metals are required for all forms of life on earth. The high reactivity of these elements means that an array of mechanisms has evolved to regulate key processes governing their transport and binding action. Tracking metals within biological tissue is non-trivial; tagging approaches suffer from lack of specificity, and can fail to find strongly-bound species; in addition, tags can interfere with normal biochemistry. Electron microscopy provides stupendous resolution, but probes miniscule volumes due to the short penetration of electrons. With μM sensitivity, X-ray Fluorescence Microscopy (XFM) can probe endogenous metal concentrations at resolutions at the μm length scale. Elemental maps are quantitative. With penetration depth and depth of field well matched at around 0.5 mm, the method can be up-scaled to 3-D visualisations via tomography. Here we report on our application of X-ray fluorescence tomography of Zn, Cu, Fe, and Mn in C. elegans and discuss recent progress in developing self-absorption corrections that will enable accurate mapping of light elements.en_AU
dc.identifier.citationde Jonge, M. D., Ruben, G., Mayo, S., Ryan, C. G., Kirkham. R., Howard, D. L., & Paterson, D. (2016). Direct imaging of endogenous biometal distributions within millimetre-scale organisms at micrometre resolution – x-ray fluorescence tomography. Paper presented to ACMM24 : Australian Conference on Microscopy and Analysis : Melbourne Convention and Exhibition Centre, 31 Jan-4 Feb 2016, (pp. 78).en_AU
dc.identifier.conferenceenddate4 February 2016en_AU
dc.identifier.conferencenameACMM24 : Australian Conference on Microscopy and Analysisen_AU
dc.identifier.conferenceplaceMelbourne, Australiaen_AU
dc.identifier.conferencestartdate31 January 2016en_AU
dc.identifier.isbn9780980337334en_AU
dc.identifier.pagination78en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14535en_AU
dc.language.isoenen_AU
dc.publisherAustralian Microscopy and Microanalysis Societyen_AU
dc.subjectMetalsen_AU
dc.subjectEarth planeten_AU
dc.subjectElectronsen_AU
dc.subjectElectron microscopyen_AU
dc.subjectX-ray fluorescence analysisen_AU
dc.subjectDepthen_AU
dc.subjectZincen_AU
dc.subjectCopperen_AU
dc.subjectIronen_AU
dc.subjectManganeseen_AU
dc.titleDirect imaging of endogenous biometal distributions within millimetre-scale organisms at micrometre resolution – x-ray fluorescence tomographyen_AU
dc.typeConference Abstracten_AU
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