Characterisation of peripheral benzodiazepine receptors changes in early phase of epileptogenesis in the rat with PET and [18F]PBR111

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dc.contributor.authorGrégoire, MCen_AU
dc.contributor.authorDedeurwaerdere, Sen_AU
dc.contributor.authorCallaghan, PDen_AU
dc.contributor.authorLoc'h, Cen_AU
dc.contributor.authorPham, TQen_AU
dc.contributor.authorKatsifis, Aen_AU
dc.date.accessioned2023-02-06T23:24:14Zen_AU
dc.date.available2023-02-06T23:24:14Zen_AU
dc.date.issued2009-05en_AU
dc.date.statistics2022-09-28en_AU
dc.descriptionCitation shows author as C. Loch. The correct spelling is Loc'h.en_AU
dc.description.abstractThe current project aims at investigating the role of microglial activation in epileptogenesis by estimating PBR changes in the rat brain with a new and highly specific PBR ligand, [18F]PBR111, during the early phase, i.e. one week after status epilepticus induced by kainic acid (KA). Methods Four controls and 6 KA rats were scanned for 180 minutes with our small animal PET/CT under a triple-injection protocol. The results presented here cover only the first 60 minutes after injection of 0.06nmoles of [18F]PBR111. Arterial blood samples were withdrawn and metabolite corrected plasma time-activity curves were derived. Each CT and PET data were co-registered to an age and strain matched rat brain atlas containing 25 Regions-Of-Interest (ROIs). The Volume of Distribution (Vd) were calculated for each region. Whole-brain-normalised Vd (nVd) were also calculated to assess local variations. Results Compared to controls, KA-treated rats show a 2 to 3-fold increase of the Vd in all brain areas except the midbrain. No significant asymmetry was detected (< 9%), so the ROIs were grouped. Normalized Vd values (Table 1) show that Amygdala, Striata, Thalamus and Hippocampus are predominantly involved. No significant changes were detected in the midbrain and all cortical areas. Estimates of receptors densitiy (Bmax) and apparent affinity (KdVr) will be calculated from the full kinetics (three phases) and reported later. Conclusions This in vivo imaging study has identified significant microglial activation during early epileptogenesis in several brain regions which are known to play a key role in chronic epilepsy.en_AU
dc.identifier.citationGregoire, M.-C., Dedeurwaerdere, S., Callaghan, P., Loch, C., Pham, T., & Katsifis, A. (2009). Characterisation of peripheral benzodiazepine receptors changes in early phase of epileptogenesis in the rat with PET and [18F]PBR111. Paper presented to the Society of Nuclear Medicine Annual Meeting, Toronto Metro Convention Center, Canada, 13-17 June, 2009. In Journal of Nuclear Medicine, 50(supplement 2), 550. Retrieved from http://jnm.snmjournals.org/content/50/supplement_2/550.abstracten_AU
dc.identifier.conferenceenddate17 June 2009en_AU
dc.identifier.conferencenameSociety of Nuclear Medicine Annual Meetingen_AU
dc.identifier.conferenceplaceToronto, Canadaen_AU
dc.identifier.conferencestartdate13 June 2009en_AU
dc.identifier.issn2159-662Xen_AU
dc.identifier.issueS2en_AU
dc.identifier.journaltitleJournal of Nuclear Medicineen_AU
dc.identifier.pagination550en_AU
dc.identifier.urihttps://jnm.snmjournals.org/content/50/supplement_2/550en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14611en_AU
dc.identifier.volume50en_AU
dc.language.isoenen_AU
dc.publisherSociety of Nuclear Medicineen_AU
dc.subjectPositron computed tomographyen_AU
dc.subjectReceptorsen_AU
dc.subjectRatsen_AU
dc.subjectBrainen_AU
dc.subjectCAT scanningen_AU
dc.subjectInjectionen_AU
dc.subjectMetabolitesen_AU
dc.subjectBlood plasmaen_AU
dc.titleCharacterisation of peripheral benzodiazepine receptors changes in early phase of epileptogenesis in the rat with PET and [18F]PBR111en_AU
dc.typeConference Paperen_AU
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