IFN-γ-induced signal-on fluorescence aptasensors: from hybridization chain reaction amplification to 3D optical fiber sensing interface towards a deployable device for cytokine sensing

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dc.contributor.authorZhang, FYen_AU
dc.contributor.authorDeng, Fen_AU
dc.contributor.authorLiu, GJen_AU
dc.contributor.authorMiddleton, RJen_AU
dc.contributor.authorInglis, DWen_AU
dc.contributor.authorAnwer, Aen_AU
dc.contributor.authorWang, Sen_AU
dc.contributor.authorLiu, GZen_AU
dc.date.accessioned2022-08-29T01:55:49Zen_AU
dc.date.available2022-08-29T01:55:49Zen_AU
dc.date.issued2019-04-29en_AU
dc.date.statistics2022-07-04en_AU
dc.description.abstractInterferon-gamma (IFN-γ), a proinflammatory cytokine, has been used as an early indicator of multiple infectious diseases or tumors. In order to explore the detection capability of a commonly used anti-IFN-γ aptamer, a simple target induced strand-displacement aptasensing strategy was tested by introducing three different complementary strands and two different signal/quencher pairs. The Texas red/BHQ2-based sensor showed the best affinity constant (Kd) of 21.87 ng mL−1. It was found that the strand-displacement aptasensing strategy was impacted by the complementary position and length of the complementary strands. Additionally, the hybridization chain reaction (HCR) amplification strategy was introduced, yielding a 12-fold improved sensitivity of 0.45 ng mL−1. In order to further explore the sensing platform for spatially localized cytokine detection, the Texas red/BHQ2-based strand-displacement aptasensor was successfully fabricated on the 3D optical fiber surface to achieve a deployable sensing device for monitoring IFN-γ based on the fluorescence spots counting strategy. Finally, the three developed aptasensing strategies (strand-displacement strategy, HCR amplification strategy, 3D optical fiber aptasensor) were applied for detection of IFN-γ secreted by PBMCs with comparable results to those of ELISA. The deployable 3D optical fiber aptasensor with the superior sensitivity is potential to be used for detection of spatially localized IFN-γ in vivo. © 2019 The Royal Society of Chemistryen_AU
dc.description.sponsorshipThis work was financially supported by funding from the ARC Future Fellowship (FT160100039), the National Natural Science Foundation of China (Grant 21575045), and the ARC Centre of Excellence for Nanoscale BioPhotonics (CE140100003). F. Z. was supported by the Macquarie University Cotutelle-iMQRTP scholarship (2017501) and Residential Student Scholarship (RSS) funded by Australian Institute of Nuclear Science and Engineering (AINSE).en_AU
dc.identifier.citationZhang, F., Deng, F., Liu, G.-J., Middleton, R., Inglis, D. W., Anwer, A., Wang, S., & Liu, G. (2019). IFN-γ-induced signal-on fluorescence aptasensors: from hybridization chain reaction amplification to 3D optical fiber sensing interface towards a deployable device for cytokine sensing. Molecular Systems Design & Engineering, 4(4), 872-881. doi:10.1039/C9ME00047Jen_AU
dc.identifier.issn2058-9689en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleMolecular Systems Design & Engineeringen_AU
dc.identifier.pagination872-881en_AU
dc.identifier.urihttps://doi.org/10.1039/C9ME00047Jen_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/13639en_AU
dc.identifier.volume4en_AU
dc.language.isoenen_AU
dc.publisherRoyal Society of Chemistryen_AU
dc.subjectFluorescenceen_AU
dc.subjectChain reactionsen_AU
dc.subjectHybridizationen_AU
dc.subjectOptical fibersen_AU
dc.subjectInterferonen_AU
dc.subjectLymphokinesen_AU
dc.titleIFN-γ-induced signal-on fluorescence aptasensors: from hybridization chain reaction amplification to 3D optical fiber sensing interface towards a deployable device for cytokine sensingen_AU
dc.typeJournal Articleen_AU
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