Neutron irradiation of human melanoma cells

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dc.contributor.authorBrown, Ken_AU
dc.contributor.authorMountford, MHen_AU
dc.contributor.authorAllen, BJen_AU
dc.contributor.authorMishima, Men_AU
dc.contributor.authorIchihashi, Men_AU
dc.contributor.authorParsons, Pen_AU
dc.date.accessioned2025-11-21T06:50:48Zen_AU
dc.date.available2025-11-21T06:50:48Zen_AU
dc.date.issued1989en_AU
dc.date.statistics2025-11-12en_AU
dc.description.abstractThe biological characteristics and in vitro radiosensitivity of melanoma cells to thermal neutrons were investigated as a guide to the effectiveness of boron neutron capture therapy. Plateau phase cultures of three human malignant melanoma-established cell lines were examined for cell density at confluence, doubling time, cell cycle parameters, chromosome constitution, and melanin content. Cell survival dose-response curves, for cells preincubated in the presence or absence of p-boronophenylalanine. HCl (10B1-BPA), were measured over the dose range 0.6-8.0 Gy (N + gamma). The neutron fluence rate was 2.6 x 10(9) n/cm2/s and the total dose rate 3.7 Gy/h (31% gamma). Considerable differences were observed in the morphology and cellular properties of the cell lines. Two cell lines (96E and 96L) were amelanotic, and one was melanotic (418). An enhanced killing for neutron irradiation was found only for the melanotic cells after 20 h preincubation with 10 micrograms/ml 10B1-BPA. In view of the doubling times of the cell lines of about 23 h (96E and 96L) or of 36 h (418), it seems likely that an increased boron uptake, and hence increased radiosensitivity, might result if the preincubation period with 10B1-BPA is extended to several hours longer than the respective cell cycle times. © Alan R. Liss Inc.en_AU
dc.description.sponsorshipPhysical copy held by ANSTO Library at DDC: 616.99477/3 Also held as part of a journal Pigment Cell Research Vol. 2, No. 4 (1989) at the same DDC number.en_AU
dc.identifier.booktitleMelanoma neutron capture therapy : proceedings of 2nd Japan-Australia International Workshop on Thermal Neutron Capture Therapy for Malignant Melanoma, Kobe, September 23-26, 1987en_AU
dc.identifier.citationBrown, K., Mountford, M. H., Allen, B. J., Mishima, Y., Ichihashi, M., & Parsons, P. (1989). Neutron irradiation of human melanoma cells. Paper prsented to the 2nd Japan-Australia International Workshop on Thermal Neutron Capture Therapy for Malignant Melanoma, Kobe, September 23-26, 1987. In Mishima, Y. (ed.). Melanoma neutron capture therapy : proceedings of 2nd Japan-Australia International Workshop on Thermal Neutron Capture Therapy for Malignant Melanoma, Kobe, September 23-26, 1987, (pp. 319-324). New York : Alan R. Liss.en_AU
dc.identifier.conferenceenddate1987-09-26en_AU
dc.identifier.conferencename2nd Japan-Australia International Workshop on Thermal Neutron Capture Therapy for Malignant Melanomaen_AU
dc.identifier.conferenceplaceKobeen_AU
dc.identifier.conferencestartdate1987-09-23en_AU
dc.identifier.editorsYukata Mishimaen_AU
dc.identifier.issn0893-5785en_AU
dc.identifier.pagination319-324en_AU
dc.identifier.placeofpublicationNew Worken_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/16733en_AU
dc.language.isoenen_AU
dc.publisherAlan R. Liss, Incen_AU
dc.relation.ispartofseriesSINCT-REP; 1en_AU
dc.subjectAmino acidsen_AU
dc.subjectAnimal cellsen_AU
dc.subjectAnimalsen_AU
dc.subjectAromaticsen_AU
dc.subjectBoron compoundsen_AU
dc.subjectCell cycleen_AU
dc.subjectDose-response relationshipsen_AU
dc.subjectMelanomasen_AU
dc.subjectNeutron capture therapyen_AU
dc.subjectPhenylalanineen_AU
dc.subjectRadiosensitivityen_AU
dc.subjectTumor cellsen_AU
dc.subjectRadiotherapyen_AU
dc.titleNeutron irradiation of human melanoma cellsen_AU
dc.typeConference Paperen_AU
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