Protein deuteration extending structural characterisations by small angle neutron scattering with contrast variation.

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dc.contributor.authorDuff, APen_AU
dc.date.accessioned2023-04-28T05:32:20Zen_AU
dc.date.available2023-04-28T05:32:20Zen_AU
dc.date.issued2020-11-11en_AU
dc.date.statistics2023-04-18en_AU
dc.description.abstractProtein deuteration enables unique applications of neutron scattering o the life sciences, at both low and high resolutions. In recent years, the National Deuteration Facility at ANSTO have developed and published a robust and efficient method of recombinant protein deuteration.[1] Utilising this method enables us to routinely collaborate with life scientists by removing the difficulty of biomolecule deuteration from their needs for sample preparation to make best use of neutron scattering. In this presentation I will highlight the essential role of protein deuteration in the structural characterization of previously poorly characterized “suppressor of copper sensitivity” proteins, as recently published.[2] Using this, and more recent work in progress, I will illustrate the value of small angle scattering as a complementary method to high resolution techniques so as to including disordered to-ordered transitions that are frequently the basis for functional mechanisms in life and disease. Other applications of protein deuteration, for neutron reflectometry, neutron crystallography, and nuclear magnetic resonance, will be briefly explained.en_AU
dc.identifier.citationDuff, A. (2020). Protein deuteration extending structural characterisations by small angle neutron scattering with contrast variation. Paper presented to the ANBUG-AINSE Neutron Scattering Symposium, AANSS 2020, Virtual Meeting, 11th - 13th November 2020. (pp. 16). Retrieved from: https://events01.synchrotron.org.au/event/125/contributions/3741/contribution.pdfen_AU
dc.identifier.conferenceenddate13 November 2020en_AU
dc.identifier.conferencenameANBUG-AINSE Neutron Scattering Symposium, AANSS 2020en_AU
dc.identifier.conferenceplaceVirtual Meetingen_AU
dc.identifier.conferencestartdate11 November 2020en_AU
dc.identifier.pagination16en_AU
dc.identifier.urihttps://events01.synchrotron.org.au/event/125/contributions/3741/contribution.pdfen_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14942en_AU
dc.language.isoenen_AU
dc.publisherAustralian Institute of Nuclear Science and Engineering (AINSE)en_AU
dc.subjectScatteringen_AU
dc.subjectANSTOen_AU
dc.subjectAustraliaen_AU
dc.subjectProteinsen_AU
dc.subjectSmall angle scatteringen_AU
dc.subjectCopperen_AU
dc.titleProtein deuteration extending structural characterisations by small angle neutron scattering with contrast variation.en_AU
dc.typeConference Abstracten_AU
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