ANSTO’s National Deuteration Facility: recent advancements and an overview on molecular deuteration capabilities for neutron applications

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dc.contributor.authorWilde, KLen_AU
dc.contributor.authorCagnes, MPen_AU
dc.contributor.authorDuff, APen_AU
dc.contributor.authorKlenner, MAen_AU
dc.contributor.authorKrause-Heuer, AMen_AU
dc.contributor.authorMoir, Men_AU
dc.contributor.authorRekas, Aen_AU
dc.contributor.authorRussell, RAen_AU
dc.contributor.authorYepuri, NRen_AU
dc.contributor.authorDarwish, TAen_AU
dc.date.accessioned2023-05-04T23:02:12Zen_AU
dc.date.available2023-05-04T23:02:12Zen_AU
dc.date.issued2020-11-11en_AU
dc.date.statistics2023-04-26en_AU
dc.description.abstractThe National Deuteration Facility (NDF) at the Australian Nuclear Science and Technology Organisation (ANSTO) provides deuteration through both biological and chemical techniques for a diversity of molecules and applications and is the only facility of its type in the Southern Hemisphere with the specialised expertise and infrastructure for both biological and chemical molecular deuteration. Molecular deuteration of organic compounds and biomolecules significantly increases the options in complex structure function investigations using neutron scattering and reflectometry, nuclear magnetic resonance (NMR), mass spectrometry (MS) and other techniques. Deuteration (substitution of the naturally occurring hydrogen stable isotope deuterium (2H or D) for 1H (or H)) can provide contrast and improved resolution to assist investigations into the relationship between molecular structure and function of molecules of both biological and synthetic origin. By developing a suite of capabilities in both in vivo deuteration of biomolecules and chemical deuteration of small organic molecules, the NDF provides access to a broad range of deuterated molecules for research and industry. Variably deuterated proteins can be produced via recombinant expression in Escherichia coli and other microbial systems utilised to produce deuterated cellulose and cholesterol. By tailoring deuteration approaches with the ongoing development of chemical deuteration protocols for a broader range of molecular classes than available commercially, the NDF has increased the range of systems that can be investigated using deuterated molecules. Lipids, phospholipids (including head or tail or head/tail deuterated mono-unsaturated lipids such as POPC and DOPC), heterocyclics, aromatics, surfactants, ionic liquids, saturated and unsaturated fatty acids, sugars and match-out detergents have been deuterated. Common neutron applications include partially deuterated proteins for SANS experiments investigating multiprotein systems, neutron crystallography of perdeuterated proteins, neutron reflectometry of lipid bilayers systems and SANS using saturated lipid vesicles, or detergents amongst others. An overview and update on the NDF will be provided which will include details on the NDF User Program (e.g. information on the available modes of access), recent advancements in custom deuterated molecules available and brief highlights of deuterated molecule utilisation for neutron experiments at ANSTO’s Australian Centre for Neutron Scattering (ACNS). © 2020 The Authors.en_AU
dc.identifier.citationWilde, K., Cagnes, M., Duff, A., Klenner, M., Krause-Heuer, A., Moir, M., Rekas, A., Russell, R., Yepuri, R., & Darwish, T. (2020). ANSTO’s National Deuteration Facility: recent advancements and an overview on molecular deuteration capabilities for neutron applications. Paper presented to the ANBUG-AINSE Neutron Scattering Symposium, AANSS 2020, Virtual Meeting, 11th - 13th November 2020, (pp. 57). Retrieved from: https://events01.synchrotron.org.au/event/125/attachments/725/1149/AANSS_Abstract_Booklet_Complete_-_1_Page_Reduced.pdfen_AU
dc.identifier.conferenceenddate13 November 2020en_AU
dc.identifier.conferencenameANBUG-AINSE Neutron Scattering Symposium, AANSS 2020en_AU
dc.identifier.conferenceplaceVirtual Meetingen_AU
dc.identifier.conferencestartdate11 November 2020en_AU
dc.identifier.pagination57en_AU
dc.identifier.urihttps://events01.synchrotron.org.au/event/125/contributions/3790/contribution.pdfen_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/14987en_AU
dc.language.isoenen_AU
dc.publisherAustralian Institute of Nuclear Science and Engineering (AINSE)en_AU
dc.subjectANSTOen_AU
dc.subjectDeuterationen_AU
dc.subjectMoleculesen_AU
dc.subjectSouthern Hemisphereen_AU
dc.subjectNeutronsen_AU
dc.subjectScatteringen_AU
dc.subjectSpectrophotometryen_AU
dc.subjectNuclear magnetic resonanceen_AU
dc.subjectEscherichia colien_AU
dc.subjectCelluloseen_AU
dc.subjectProteinsen_AU
dc.subjectCrystallographyen_AU
dc.subjectHeterocyclic acidsen_AU
dc.subjectAromaticsen_AU
dc.titleANSTO’s National Deuteration Facility: recent advancements and an overview on molecular deuteration capabilities for neutron applicationsen_AU
dc.typeConference Abstracten_AU
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