Protein perdeuteration for neutron crystallography

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dc.contributor.authorDuff, APen_AU
dc.date.accessioned2020-11-12T05:37:28Zen_AU
dc.date.available2020-11-12T05:37:28Zen_AU
dc.date.issued2019-03-28en_AU
dc.date.statistics2020-10-30en_AU
dc.description.abstractProtein deuteration by recombinant expression in deuterated minimal media offers advantages to several experimental techniques involving neutron scattering. Perdeuteration improves neutron crystallographic studies of proteins. Although not required, protein perdeuteration offers advantages regarding crystal size, data collection time, and the clarity of the resulting experimental nuclear density maps. The level of advantage of perdeuteration depends on practical matters of the existing method of protein production. If recombinant expression yields are high, and purification is efficient, the staff and consumable costs for protein perdeuration are not so great. ©2019 The Physical Society of Japan under the terms of the Creative Commons Attribution 4.0 Licenseen_AU
dc.identifier.articlenumber11003en_AU
dc.identifier.articlenumberISQBSS2018en_AU
dc.identifier.citationDuff, A. P. (2019). Protein perdeuteration for neutron crystallography. Paper presented to the 3rd International Symposium of Quantum Beam Science at Ibaraki University, Japan, May 30 - June 2 2018, "Quantum Beam Science in Biology and Soft Materials (ISQBSS2018). In Journal of the Physical Society of Japan, 25, ISQBSS2018. doi:10.7566/JPSCP.25.011003en_AU
dc.identifier.conferenceenddate2 June 2018en_AU
dc.identifier.conferencename3rd International Symposium of Quantum Beam Scienceen_AU
dc.identifier.conferenceplaceIbaraki, Japanen_AU
dc.identifier.conferencestartdate30 May 2018en_AU
dc.identifier.isbn978-4-89027-135-1en_AU
dc.identifier.issue25en_AU
dc.identifier.journaltitleJournal of the Physical Society of Japanen_AU
dc.identifier.urihttps://doi.org/10.7566/JPSCP.25.011003en_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/9982en_AU
dc.identifier.volume25en_AU
dc.language.isoenen_AU
dc.publisherThe Physical Society of Japanen_AU
dc.subjectProtein denaturationen_AU
dc.subjectDeuterationen_AU
dc.subjectNeutronsen_AU
dc.subjectCrystallographyen_AU
dc.subjectProteinsen_AU
dc.subjectYieldsen_AU
dc.subjectChemical reactionsen_AU
dc.titleProtein perdeuteration for neutron crystallographyen_AU
dc.title.alternativeProceedings of the 3rd International Symposium of Quantum Beam Science at Ibaraki University "Quantum Beam Science in Biology and Soft Materials (ISQBSS2018)"en_AU
dc.typeConference Paperen_AU
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