Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron‐sourced terahertz radiation

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dc.contributor.authorPerera, PGTen_AU
dc.contributor.authorVilagosh, Zen_AU
dc.contributor.authorLinklater, Den_AU
dc.contributor.authorNguyen, THPen_AU
dc.contributor.authorAppadoo, DRTen_AU
dc.contributor.authorVongsvivut, JPen_AU
dc.contributor.authorTobin, MJen_AU
dc.contributor.authorDekiwadia, Cen_AU
dc.contributor.authorCroft, Ren_AU
dc.contributor.authorIvanova, EPen_AU
dc.date.accessioned2025-10-24T04:23:07Zen_AU
dc.date.available2025-10-24T04:23:07Zen_AU
dc.date.issued2023-07-01en_AU
dc.date.statistics2025-10-01en_AU
dc.description.abstractThe routes by which foreign objects enter cells is well studied; however, their fate following uptake has not been explored extensively. Following exposure to synchrotron-sourced (SS) terahertz (THz) radiation, reversible membrane permeability has been demonstrated in eukaryotic cells by the uptake of nanospheres; nonetheless, cellular localization of the nanospheres remained unclear. This study utilized silica core-shell gold nanospheres (AuSi NS) of diameter 50 ± 5 nm to investigate the fate of nanospheres inside pheochromocytoma (PC 12) cells following SS THz exposure. Fluorescence microscopy was used to confirm nanosphere internalization following 10 min of SS THz exposure in the range 0.5–20 THz. Transmission electron microscopy followed by scanning transmission electron microscopy energy-dispersive spectroscopic (STEM-EDS) analysis was used to confirm the presence of AuSi NS in the cytoplasm or membrane, as single NS or in clusters (22% and 52%, respectively), with the remainder (26%) sequestered in vacuoles. Cellular uptake of NS in response to SS THz radiation could have suitable applications in a vast number of biomedical applications, regenerative medicine, vaccines, cancer therapy, gene and drug delivery. © International Union of Crystallography. Open Access CC-BY 4.0en_AU
dc.description.sponsorshipThe authors would like to acknowledge financial support of the NHMRC Centre of Research Excellence for Electromagnetic Bioeffects Research (Grant No. CRE1042464 and CRE1135076) and the funding received by Australian Radiation Protection and Safety Agency (ARPANSA) under the EME Research Framework.en_AU
dc.format.mediumPrint-Electronicen_AU
dc.identifier.citationPerera, P. G. T., Vilagosh, Z., Linklater, D., Nguyen, T. H. P., Appadoo, D., Vongsvivut, J., Tobin, M., Dekiwadia, C., Croft, R., & Ivanova, E. P. (2023). Translocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron-sourced terahertz radiation. Journal of Synchrotron Radiation, 30(4), 780–787. doi:10.1107/S1600577523004228en_AU
dc.identifier.issn0909-0495en_AU
dc.identifier.issn1600-5775en_AU
dc.identifier.issue4en_AU
dc.identifier.journaltitleJournal of Synchrotron Radiationen_AU
dc.identifier.pagination780-787en_AU
dc.identifier.urihttps://doi.org/10.1107/s1600577523004228en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/16648en_AU
dc.identifier.volume30en_AU
dc.languageengen_AU
dc.language.isoenen_AU
dc.publisherInternational Union of Crystallographyen_AU
dc.subjectSynchrotronsen_AU
dc.subjectRadiationsen_AU
dc.subjectElectromagnetic fieldsen_AU
dc.subjectFluorescenceen_AU
dc.subjectElectron microscopyen_AU
dc.subjectSilicaen_AU
dc.subjectDrugsen_AU
dc.subjectVaccinesen_AU
dc.subjectTranslocationen_AU
dc.subjectTHz rangeen_AU
dc.subjectTransmission electron microscopyen_AU
dc.titleTranslocation and fate of nanospheres in pheochromocytoma cells following exposure to synchrotron‐sourced terahertz radiationen_AU
dc.typeJournal Articleen_AU
dcterms.dateAccepted2023-05-15en_AU
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