Examining the creation and destruction of model bacterial surfaces

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dc.contributor.authorClifton, LAen_AU
dc.contributor.authorSkoda, MWAen_AU
dc.contributor.authorHughes, Aen_AU
dc.contributor.authorHolt, SAen_AU
dc.contributor.authorLakey, Jen_AU
dc.date.accessioned2021-08-17T02:28:37Zen_AU
dc.date.available2021-08-17T02:28:37Zen_AU
dc.date.issued2017-07-12en_AU
dc.date.statistics2021-08-10en_AU
dc.description.abstractBacteria are differentiated into two main groups, Gram-positive or Gram-negative, based on the Gram stain which detects the thick peptidoglycan cell wall of gram positive bacteria.Gram-negative bacteria are of particular biomedical and technological interest due to their role in disease, the increasing antibiotic resistance if some species and their utility in many biotechnological processes. The Gram-negative bacterial outer membrane (GNB-OM) is asymmetric in its lipid composition with a phospholipid-rich inner leaflet and an outer leaflet predominantly composed of lipopolysaccharides (LPS). LPS is a polyanionic molecule, with numerous phosphate groups present in the Lipid A and core oligosaccharide regions. We have attempted to create GNB-OM assays which are amenable to molecular level characterisation. These systems are asymmetric phospholipid : lipopolysaccharide membranes deposited at the solid/liquid interface and consist of either solid supported bilayers at the silicon/water interface or floating supported bilayers at the gold/water interface. The analysis of these membrane models by neutron reflectometry has provided new insights into the OM and interactions with it. Examples of this include providing conformation of the activity of anti-bacterial proteins, the role of the lipopolysaccharide polysaccharide chains in protecting the bacterium and the importance of divalent cations in stabilising the OM structure.en_AU
dc.identifier.citationClifton, L., Skoda, M., Hughes, A., Holt, S., & Lakey, J. (2017). Examining the creation and destruction of model bacterial surfaces. Paper presented at ICNS 2017 (International Conference on Neutron Scattering), Daejeon, South Korea, 9 to 13 July 2017. Retrieved from: http://www.icns2017.org/program.phpen_AU
dc.identifier.conferenceenddate13 July 2017en_AU
dc.identifier.conferencenameICNS 2017 (International Conference on Neutron Scattering)en_AU
dc.identifier.conferenceplaceDaejeon, South Koreaen_AU
dc.identifier.conferencestartdate9 July 2017en_AU
dc.identifier.uriRetrieved from: http://www.icns2017.org/program.phpen_AU
dc.identifier.urihttps://apo.ansto.gov.au/dspace/handle/10238/11374en_AU
dc.language.isoenen_AU
dc.publisherInternational Conference on Neutron Scatteringen_AU
dc.subjectBacteriaen_AU
dc.subjectPhospholipidsen_AU
dc.subjectLipopolysaccharidesen_AU
dc.subjectPeptidesen_AU
dc.subjectOligosaccharidesen_AU
dc.subjectNeutron diffractionen_AU
dc.titleExamining the creation and destruction of model bacterial surfacesen_AU
dc.typeConference Abstracten_AU
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