Oxidative damage to biomimetic membrane systems: In situ Fe(II)/ascorbate initiated oxidation and incorporation of synthetic oxidized phospholipids

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dc.contributor.authorKnobloch, JJen_AU
dc.contributor.authorNelson, ARJen_AU
dc.contributor.authorKöper, Ien_AU
dc.contributor.authorJames, Men_AU
dc.contributor.authorMcGillivray, DJen_AU
dc.date.accessioned2025-08-08T03:39:31Zen_AU
dc.date.available2025-08-08T03:39:31Zen_AU
dc.date.issued2015-10-30en_AU
dc.date.statistics2025-03-19en_AU
dc.description.abstractDamage to cellular membranes from oxidative stress has been implicated in aging related diseases. We report the effects of oxidative damage on the structure and properties of biomimetic phospholipid membrane systems. Two oxidation methods were used, in situ oxidation initiated using Fe(II) and ascorbate, and the incorporation of a synthetic "oxidized" phospholipid, PoxnoPC, into biomimetic membranes. The biomimetic systems employed included multibilayer stacks, tethered bilayers, and phospholipid monolayers studied using a combination of reflectometry, attenuated total reflection infrared spectroscopy, electrochemical impedance spectroscopy, and neutron diffraction. We show that oxidation with Fe(II) and ascorbate caused an increase in the order of the membrane, attributed to cross-linking of the phospholipids, and a change in the electrical permeability of the membrane, but no significant impact on the thickness or completeness of the membrane. Incorporation of PoxnoPC, on the other hand, had a larger impact on the structure of the membrane. Inversion of the aldehyde-terminated truncated sn-2 chain of PoxnoPC into the head group region was observed, along with a slight decrease in the thickness and order of the membrane. © 2015 American Chemical Society.en_AU
dc.format.mediumPrint-Electronicen_AU
dc.identifier.citationKnobloch, J. J., Nelson, A. R. J., Köper, I., James, M., & McGillivray, D. J. (2015). Oxidative damage to biomimetic membrane systems: In situ Fe(II)/ascorbate initiated oxidation and incorporation of synthetic oxidized phospholipids. Langmuir, 31(46), 12679-12687. doi:10.1021/acs.langmuir.5b02458en_AU
dc.identifier.issn0743-7463en_AU
dc.identifier.issn1520-5827en_AU
dc.identifier.issue46en_AU
dc.identifier.journaltitleLangmuiren_AU
dc.identifier.pagination12679-12687en_AU
dc.identifier.urihttps://doi.org/10.1021/acs.langmuir.5b02458en_AU
dc.identifier.urihttps://apo.ansto.gov.au/handle/10238/16393en_AU
dc.identifier.volume31en_AU
dc.languageEnglishen_AU
dc.language.isoenen_AU
dc.publisherAmerican Chemical Society (ACS)en_AU
dc.subjectBiomimeticsen_AU
dc.subjectHydrocarbonsen_AU
dc.subjectLipidsen_AU
dc.subjectMembranesen_AU
dc.subjectOxidationen_AU
dc.subjectPhospholipidsen_AU
dc.subjectElectrochemistryen_AU
dc.subjectSpectroscopyen_AU
dc.titleOxidative damage to biomimetic membrane systems: In situ Fe(II)/ascorbate initiated oxidation and incorporation of synthetic oxidized phospholipidsen_AU
dc.typeJournal Articleen_AU
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